超高效液相色谱-质谱联用技术解析沙棘果超临界CO2萃取物中黄酮类天然产物结构  

Determination of Flavonoids in the Supercritical CO2 Extract of Sea Buckthorn Fruit by Using Ultra-High Performance Liquid Chromatography-Mass Spectrometry

作　　者：丁丽娜 邱亦亦 束彤 阮晖[1,3,4] DING Lina;QIU Yiyi;SHU Tong;RUAN Hui(College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China;Qinghai Food Inspection and Testing Institute, Xining 810008, China;Zhejiang Key Laboratory for Agro-Food Processing,Hangzhou 310058, China;Ningbo Research Institute, Zhejiang University, Ningbo 315100, China)

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [2]青海省食品检验检测院,青海西宁810008 [3]浙江省农产品加工技术研究重点实验室,浙江杭州310058 [4]浙江大学宁波研究院,浙江宁波315100

出　　处：《食品科学》2019年第18期273-280,共8页Food Science

基　　金：浙江省科技计划项目（2017C26004）;青海省科技计划项目（2017-ZJ-Y06）

摘　　要：采用超临界CO2萃取技术获得沙棘果油,通过超高效液相色谱-质谱联用技术和Peakview色谱工作站拟合、二级质谱解析和紫外光谱及质谱数据比对,确定有关化合物的组成和分子结构。共鉴定出沙棘果超临界CO2萃取物中含有18 种黄酮类化合物,其中包括8 种黄酮醇苷元及黄酮醇苷,3 种二氢黄酮醇苷元及二氢黄酮醇苷,5 种黄酮苷元及黄酮苷,1 种二氢黄酮苷元和1 种黄烷醇苷,其中芹菜素-6-C-葡萄糖苷-8-C-木糖苷、儿茶素-7-吡喃葡萄糖苷、苜蓿素、紫罗兰素、刺槐黄素这5 种黄酮首次被发现存在于沙棘中。Sea buckthorn fruit oil was obtained by supercritical CO 2 extraction, and its flavonoid components were analyzed by ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) through Peakview workstation matching followed by tandem mass spectrometric analysis and comparison of ultraviolet spectroscopic data and mass spectral data with those of reference standards as well as with literature data. A total of 18 flavonoid compounds were determined, including eight flavonol aglycones and flavonol glycosides, three dihydroflavonol aglycones and dihydroflavonol glycosides, five flavonoid aglycones and flavonoid glycosides, one dihydroflavonoid aglycon and one flavanol glycoside. To our knowledge, apigenin-6-C-glucoside-8-C-xyloside, catechin-7-glucopyranoside, alizarin, violet and locustin were found in sea buckthorn for the fi rst time.

关 键 词：沙棘 黄酮 超临界CO2萃取技术 超高效液相色谱-质谱联用 

分 类 号：TS201.2[轻工技术与工程—食品科学]