复合酶法提取白雪茶多糖工艺研究  

Optimization of complex enzyme extracted process of polysaccharide from Thamnolia spp.

作　　者：祁文露 肖聪 赵艳 李学颖 杨青松 QI Wen-lu;XIAO Cong;ZHAO Yan;LI Xue-ying;YANG Qing-song(School of Ethnomedicine and Ethnopharmacy Yunnan Minzu University/Key Laboratory of Ethnic Medicine Resource Chemistry,State Ethnic Affairs Commission & Ministry of Education,Kunming 650504,China;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南民族大学民族医药学院/民族药资源化学国家民委-教育部重点实验室,昆明650504 [2]云南农业大学农学与生物技术学院,昆明650201

出　　处：《湖北农业科学》2019年第17期104-108,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目（31460137;81760694）;云南省教育厅基金项目（2014C070Y）;云南农业大学自然科学青年基金项目(2015ZR15)

摘　　要：以民族药白雪茶为材料,多糖提取率为检测指标,在单因素试验基础上,设计正交试验确定复合酶法提取多糖的最佳工艺条件,并测定多糖在体外清除DPPH和亚硝酸盐能力。结果表明,在复合酶为纤维素酶和木瓜蛋白酶(质量比1∶4)条件下,酶解温度50℃,酶解时长1.0h,pH5.0,酶浓度3.0%,白雪茶多糖提取为10.81%。白雪茶多糖对亚硝酸盐和DPPH自由基均有一定的清除作用,且表现为剂量效应关系。当白雪茶多糖浓度为2.5mg/mL时,对DPPH自由基的清除率达98%。多糖浓度为2.0mg/mL时,对亚硝酸钠的清除率达19.8%。采用复合酶法提取白雪茶多糖,可以明显提高多糖的提取率,且该工艺提取的白雪茶多糖对DPPH自由基有很好的清除作用。Based on single factor experiment,the optimum conditions for the complex enzyme extraction of polysaccharides from the ethnodrug Thamnolia spp.was determined by an orthogonal experiments and analysis of variance taking extraction rate as index.Then the ability of polysaccharides to scavenge DPPH and nitrite was determined in vitro too.The results as follow: the extraction rate of Thamnolia spp.polysaccharides was the highest in the case of complex enzymes of cellulase and papain (mass ratio 1∶4),the enzymolysis temperature at 50 ℃,the enzymolysis time in 1.0 h,pH value at 5.0,and the enzyme concentration in 3.0%.And in these case,the total sugar yield is 10.8%.The Thamnolia spp.polysaccharide extracted by this condition had a certain scavenging effect on nitrite and DPPH free radicals,and showed a dose-effect relationship.When Thamnolia spp.polysaccharide concentration was 2.5 mg/mL,the DPPH radical scavenging rate was 98%.When the polysaccharide concentration was 2.0 mg/mL,the scavenging rate of sodium nitrite was 19.8%.The results showed that the extraction of polysaccharide from Thamnolia spp.by complex enzyme method can significantly increase the extraction rate of polysaccharides.And the polysaccharides extracted from this technology from Thamnolia spp.had good DPPH free radical scavenging ability.

关 键 词：复合酶法 白雪茶 多糖 提取 正交试验 

分 类 号：S571.1[农业科学—茶叶生产加工] R284.2[农业科学—作物学]