离子色谱法测定低乳糖和无乳糖牛乳中乳糖含量  

Determination of Lactose in Low-Lactose Milk and Lactose-Free Milk by Ion Chromatography

作　　者：胡雪 武伦玮 刘丽君 李翠枝 吕志勇 段国霞 岳虹 陈静 刘春霞 赵静 HU Xue;WU Lunwei;LIU Lijun;LI Cuizhi;Lü Zhiyong;DUAN Guoxia;YUE Hong;CHEN Jing;LIU Chunxia;ZHAO Jing(Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010110, China)

机构地区：[1]内蒙古伊利实业集团股份有限公司

出　　处：《乳业科学与技术》2019年第4期21-24,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

摘　　要：建立低乳糖和无乳糖牛乳中乳糖含量的离子色谱测定法.用3%冰乙酸作为牛乳蛋白沉淀剂,CarboPacTM PA20分析柱分离乳糖,采用梯度洗脱程序进行洗脱,电化学检测器检测,外标法定量.结果表明:乳糖的检出限为50 mg/kg,定量限为100 mg/kg;当牛乳中乳糖添加量为100~500 mg/kg时,回收率为92.3%~103.4%,相对标准偏差为1.44%~4.43%.该方法具有快速、准确、重复性好、灵敏度高及操作简便的特点.A method for the determination of lactose in low-lactose milk and lactose-free milk by using ion chromatography was developed. Lactose from samples was extracted with 3% acetic acid as a protein precipitator. The method was performed on a CarboPacTM PA20 column by gradient elution. The analyte was detected with an electrochemical detector and quantified by an external standard method. The limit of quantification was 100 mg/kg and the limit of detection was 50 mg/kg for lactose in milk. The recoveries at spiked concentration levels of 100–500 mg/kg were 92.3%–103.4%, with relative standard deviations between 1.44% and 4.43%. This method proved to be rapid, accurate, repeatable, sensitive and simple.

关 键 词：低乳糖牛乳 无乳糖牛乳 离子色谱法 乳糖 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]