核桃中血清素的提取分离与抗心肌缺血活性研究  

Extraction and isolation of serotonin from walnut and its anti-ischemic activity

作　　者：陈晨[1] 徐德平[1] 金蒙 张晓娟 CHEN Chen;XU De-ping;JIN Meng;ZHANG Xiao-juan(Food Faculty of Jiangnan University, Wuxi, Jiangsu 214122, China)

机构地区：[1]江南大学食品学院

出　　处：《食品与机械》2019年第8期167-171,共5页Food and Machinery

基　　金：江苏高校品牌专业建设工程资助项目(编号:PPZY2015A052);云南省重大科技专项计划项目(编号:2018ZG003)

摘　　要：核桃破碎后经体积分数为70%乙醇提取,乙酸乙酯萃取、浓缩后,经大孔树脂、MCI、ODS等不同层析柱分离。采用MTT法和试剂盒法,以乳鼠心肌细胞缺血/再灌注损伤模型检测各分离组分抗心肌缺血活性。结果从核桃中分离得到5,10-二羟色胺和Shepherdine化合物,其中5,10-二羟色胺能显著提高心肌细胞存活率,减少受损细胞心肌酶(LDH、CK)的释放,对心肌细胞具有保护作用。The functional components of anti-myocardial ischemic activity in walnut were studied.The walnuts were squashed and then extracted by 70%ethanol,and ethyl acetate.The ethyl acetate phase was concentrated and then separated by macroporous resin,MCI,and ODS columns.MTT assay and kit method were applied,along with the model of myocardial ischemia/reperfusion injury to detect the anti-ischemic activity of each isolated component.The results showed that 5,10-dihydroxytryptamine and Shepherdine were separated from walnuts for the first time.Moreover,5,10-dihydroxytryptamine could significantly maintain the viability of myocardial cell and reduce the enzymes(LDH/CK)released by ischemia/reperfusion injury,having significant protective effects on myocardial ischemia/reperfusion injury.

关 键 词：核桃 血清素 5 10-二羟色胺 心肌细胞 缺血/再灌注 

分 类 号：TS201.2[轻工技术与工程—食品科学]