Bupleurum polysaccharides ameliorated renal injury in diabetic mice associated with suppression of HMGB1-TLR4 signaling  

作　　者：LIU Zhen-Zhen WENG Hong-Bo ZHANG Li-Jie PAN Ling-Yu SUN Wei CHEN Hai-Xia CHEN Mei-Yu ZENG Tao ZHANG Yun-Yi CHEN Dao-Feng LI Hong 

机构地区：[1]Department of Pharmacology,School of Pharmacy,Fudan University,Shanghai 201203,China [2]Department of Pharmacognosy,School of Pharmacy,Fudan University,Shanghai 201203,China [3]Clinical trial institution,Obstetrics and Gynecology Hospital of Fudan University,Shanghai 201203,China

出　　处：《Chinese Journal of Natural Medicines》2019年第9期641-649,共9页中国天然药物（英文版）

基　　金：funded by the National Natural Science Foundation of China(Nos.81673658,81274165 and 30925042);Ministry of Science and Technology of China(No.2018ZX09735003-002);the Science and Technology Commission of Shanghai Municipality(No.12JC1400800)

摘　　要：Bupleurum polysaccharides(BPs)is isolated from Bupleurum smithii var.parvifolium,a key traditional Chinese medicine.The study was to investigate the effects of BPs on diabetic kidney injury.After two intraperitoneal injections of streptozotozin(STZ)100 mg·kg^–1,renal injury in diabetic mice was induced and BPs was orally administrated at dosages of 30 and 60 mg·kg^–1·d^–1.The STZ injected mice developed renal function damage,renal inflammation and fibrosis known as diabetic kidney disease(DKD).BPs significantly reduced serum creatinine level and urinary albumin excretion rate,with the attenuated swelling of kidneys.BPs treatment obviously alleviated the pathological damage of renal tissue.The progression of renal injury in BPs treated mice was inhibited with less expression of type IV collagen(Col IV),fibronectin(FN)andα-smooth muscle actin(α-SMA).The inhibition of inflammation in kidney was associated with the reduced level of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).BPs administration suppressed the over-expression of toll like receptor 4(TLR4)and high-mobility group box 1(HMGB1)with lowered activity of nuclear factor kappa B(NF-κB)in renal tissue of diabetic mice.Oral administration of BPs effectively prevented the development of renal injury in diabetic mice.This study suggested that the protection provided by BPs might affect through the interruption of HMGB1-TLR4 pathway,leading to the inhibition of renal inflammation and fibrotic process.Bupleurum polysaccharides（BPs） is isolated from Bupleurum smithii var. parvifolium, a key traditional Chinese medicine. The study was to investigate the effects of BPs on diabetic kidney injury. After two intraperitoneal injections of streptozotozin（STZ） 100 mg·kg–1, renal injury in diabetic mice was induced and BPs was orally administrated at dosages of 30 and 60 mg·kg–1·d–1. The STZ injected mice developed renal function damage, renal inflammation and fibrosis known as diabetic kidney disease（DKD）. BPs significantly reduced serum creatinine level and urinary albumin excretion rate, with the attenuated swelling of kidneys. BPs treatment obviously alleviated the pathological damage of renal tissue. The progression of renal injury in BPs treated mice was inhibited with less expression of type IV collagen（Col IV）, fibronectin（FN） and α-smooth muscle actin（α-SMA）. The inhibition of inflammation in kidney was associated with the reduced level of tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6）. BPs administration suppressed the over-expression of toll like receptor 4（TLR4） and high-mobility group box 1（HMGB1） with lowered activity of nuclear factor kappa B（NF-κB） in renal tissue of diabetic mice. Oral administration of BPs effectively prevented the development of renal injury in diabetic mice. This study suggested that the protection provided by BPs might affect through the interruption of HMGB1-TLR4 pathway, leading to the inhibition of renal inflammation and fibrotic process.

关 键 词：RENAL injury Bupleurum:High-mobility group box TOLL-LIKE receptor 4 Inflammation RENAL FIBROSIS POLYSACCHARIDES 

分 类 号：R965[医药卫生—药理学]