中华羊茅内生真菌Epichlo? sp.挥发性物质的抑菌活性及成分分析  

Antimicrobial activity and composition of volatile substance of Epichlo? sp.endophyte isolated from Festuca sinensis

作　　者：周连玉 钟松 朵红梅 乔枫 ZHOU Lian-yu;ZHONG Song;DUO Hong-mei;QIAO Feng(Key Laboratory of Medicinal Plant and Animal Resources of the Qinghai-Tibetan Plateau in Qinghai Province,School of Life Science,Qinghai Normal University;The 4 th Peoples Hospital of Qinghai Province,Xining 810008,China)

机构地区：[1]青海省青藏高原药用动植物资源重点实验室青海师范大学生命科学学院 [2]青海省第四人民医院,西宁810008

出　　处：《天然产物研究与开发》2019年第9期1543-1551,共9页Natural Product Research and Development

基　　金：国家自然科学地区科学基金(31760697);青海师范大学校级教学研究项目(qhnujy2018119,qhnujy2016136);青海省青藏高原药用动植物资源重点实验室项目(2017-ZJ-Y13)

摘　　要：为测定中华羊茅内生真菌Epichloe sp.挥发性物质的抑菌活性和成分,依次采用挥发油提取器和乙酸乙酯萃取Epichloe sp.菌株的发酵产物,再采用琼脂扩散法、悬滴法和滤纸片扩散法测定其抑菌活性;基于顶空固相微萃取和气相质谱联用法分析挥发性物质成分。结果表明:挥发性物质、乙酸乙酯提取物、水相部分和发酵液可以抑制小麦根腐离蠕孢(Bipolaris sorokiniana)、新月弯孢(Curvularia lunata)和黑曲霉(Aspergillus niger)菌丝生长。高浓度的挥发性物质对四种真菌孢子萌发和芽管长度抑制效果显著高于低浓度(P<0.05)。结果表明,随着挥发性物质浓度的增加对金黄色葡萄球菌(Staphylococcus aureus)和大肠杆菌(Escherichia coli)的抑菌作用增强,同一挥发性物质浓度对金黄色葡萄球菌的抑制作用强于大肠杆菌。此外,从挥发性物质中鉴定出32种具有较高质谱匹配度的化合物,其中主要成分包括异山梨醇(16.26%)、甲基叔丁基醚(8.18%)、二氢甘露醇(3.75%)、羟基丙酮(2.99%)、乙酸(1.22%)、己醇(1.20%)、3-糠醛(0.92%)、5-羟甲基二氢呋喃-2-酮(0.86%)、5-羟基戊酸-2,4-二叔丁基苯酯(0.86%)和棕榈酸甲酯(0.85%)。总之,中华羊茅内生真菌Epichlosp.挥发性物质具有拮抗细菌和真菌的作用。In order to investigate antimicrobial activity of volatile substance of Epichloe sp.isolated from Festuca sinensis and its components,its fermentation metabolites were partitioned by volatile oil extractor and ethyl acetate in turn and evaluated for in vitro antimicrobial activity using agar diffusion method,cavity slide method and filter paper diffusion,respectively.The volatile substance was analyzed by means of solid-phase microextraction and gas chromatography-mass spectrometry (GC-MS).The results showed that volatile substance,ethyl acetate extract,water phase and fermentation liquid had different inhibitory effects against mycelial growth of fungi Bipolaris sorokiniana,Curvularia lunata and Aspergillus niger.The high concentrations of volatile substance inhibited significantly spore germination and germ tube length of the tested fungi compared to the low concentrations ( P <0.05).In addition,diameter of inhibition zone of bacteria Staphylococcus aureus and Escherichia coli were significantly increased by volatile substance treatment when compared with control ( P <0.05),with the inhibitory effect of the same volatile substance concentration on S.aureus being more than that of E.coli.Thirty-two compositions were identified from the volatile substance of Epichlo sp.endophyte by GC-MS analysis with high similarity.The main compositions were isosorbide (16.26%),propane,2-methoxy-2-methyl-(8.18%),dianhydromannitol (3.75%),2-propanone,1-hydroxy-(2.99%),acetic acid (1.22%),1-hexanol (1.20%),3-furaldehyde (0.92%),5-hydroxymethyldihydrofuran-2-one (0.86%),pentanoic acid,5-hydroxy-,2,4-di-t-butylphenyl esters (0.86%) and hexadecanoic acid,methyl ester (0.85%).Fermentation metabolites of Epichloe sp.isolated from F.sinensis had antibacterial and antifungal activities.

关 键 词：中华羊茅 内生真菌Epichloe sp. 挥发性物质 抑菌活性 组成成分 

分 类 号：R284.2[医药卫生—中药学] Q939.99[医药卫生—中医学]