微生物酯酶的开发及其在食品工业中的应用现状  

Exploitation and application of microbial esterases in food industries

作　　者：毕文慧 桂伦[2] 姚健[2] BI Wenhui;GUI Lun;YAO Jian(College of Food Science and Engineering Shandong Agriculture and Engineering University,Jinan 250100,China;Inst.of Agric.Appl.Microbiol.,Jiangxi Acad.of Agric.Sci.,Nanchang 330200,China)

机构地区：[1]山东农业工程学院食品科学与工程学院,山东济南250100 [2]江西省农业科学院农业应用微生物研究所,江西南昌330200

出　　处：《食品与发酵工业》2019年第17期272-277,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(地区基金)(31660016);好氧堆肥高温期微生物宏基因组文库中嗜热酯酶的筛选及酶学特征研究(QNKJZ201901);肉灵芝群落结构鉴定及人工栽培条件优化(2016CQN008)

摘　　要：微生物酯酶是一种在食品领域应用广泛的工业酶,开发和应用微生物酯酶资源对食品工业发展具有重要意义。借助分离纯化、宏基因组学、宏转录组学等技术手段开发具有化学试剂抗性、热稳定性或低温耐受性的高温酯酶和低温酯酶既丰富了酶学知识又扩展了潜在工业酯酶种类;将各类酯酶用于食品加工及食品农残检测,能够有效提高生产效率、产品质量,保障食品安全。该文综述了新型微生物酯酶的开发及其在食品加工、农残检测中的应用研究进展,以期为酯酶的开发及其工业化应用提供参考。Microbial esterases are a kind of industrial enzyme that has been widely used in food industries,and it is important and beneficial for food industries to exploit and apply. Using technologies, such as isolation and purification,metagenome,metatranscriptomics,to find thermophilic esterases and psychrophilic esterases that are thermostable and tolerant to chemicals and low temperature can enrich the enzymatic knowledge and expand the variety. It has been found that applying various esterases in food production and food pesticide residue detection enhances the productivity,product quality and ensures food safety. This review summarized the state of art in exploiting novel esterases,applications in food production and pesticide residue detection in order to provide a reference for their development and industrial scale applications.

关 键 词：高温酯酶 低温酯酶 宏组学 食品加工 农残检测 

分 类 号：TS201.25[轻工技术与工程—食品科学]