鲫鱼肉中DHA和EPA的分析  

Analysis of DHA and EPA in Carp Meat

作　　者：李铁纯 侯冬岩 回瑞华 刁全平 LI Tiechun;HOU Dongyan;HUI Ruihua;DIAO Quanping(School of Chemistry and Life Science,Anshan Normal University,Anshan Liaoning 114007,China)

机构地区：[1]鞍山师范学院化学与生命科学学院

出　　处：《鞍山师范学院学报》2019年第4期42-45,共4页Journal of Anshan Normal University

摘　　要：对鲫鱼肉中DHA和EPA进行分析.采用有机溶剂提取鲫鱼肉中脂肪油,将脂肪油中的脂肪酸进行甲酯化,用气相色谱-质谱联用法鉴定鲫鱼肉中的DHA和EPA,并测定其相对百分含量.采用高效液相色谱法定量分析鲫鱼肉中DHA和EPA的含量.DHA的标准偏差为0.67%,EPA的标准偏差为0.38%.DHA的回收率为97.16%~104.20%,EPA的回收率为94.71%~101.10%.实验结果表明:气相色谱-质谱联用法测定鲫鱼肉中含DHA相对百分含量为5.33%,EPA相对百分含量为8.12%;高效液相色谱法测得鲫鱼肉含DHA18.56μg/g,含EPA89.76μg/g.DHA and EPA in carp meat were analyzed.Organic solvent was used to extract fatty oil from carp meat.Fatty acids in fatty oils were methyl esterified.DHA and EPA in carp meat were identified by gas chromatography-mass spectrometry,and their relative percentages were determined.DHA and EPA in carp meat were quantitatively analyzed by HPLC.The standard deviation of DHA was 0.67%,and the standard deviation of EPA was 0.38%.The recovery rate of DHA was 97.16%~104.20%,and that of EPA was 94.71%~101.10%.The results showed that the relative percentage of DHA and EPA in carp meat was 5.33%and 8.12%respectively by gas chromatography-mass spectrometry.The DHA18.56μg/g and EPA89.76μg/g concentrations of carp meat were determined by high performance liquid chromatography.

关 键 词：鲫鱼肉 DHA EPA 气相色谱-质谱联用 高效液相色谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]