中国健康汉族人基因组DNA中干扰素α1和干扰素α2等位基因组成的测定  

Determination of interferon alpha1 and alpha2 allele composition in the genomic DNA from Chinese healthy Han population

作　　者：王兵 杨昊欣 杨楠 鞠泳兴 卢晨 程联胜[3] 龚庆国 WANG Bing;YANG Hao-xin;YANG Nan;JU Yong-xing;LU Chen;CHENG Lian-sheng;GONG Qing-guo(The Biology Institute of Anhui Provence,Hefei 230001;Anke Precision Medicine Laboratory Limited Company of Hefei City,Hefei 230001;School of Life Science,University of Science and Technology of China,Hefei 230026,China)

机构地区：[1]安徽省生物研究所,合肥230001 [2]合肥安科精准医学检验所有限公司,合肥230001 [3]中国科学技术大学生命科学学院,合肥230026

出　　处：《生物学杂志》2019年第5期41-45,共5页Journal of Biology

摘　　要：人类干扰素α1和干扰素α2目前均发现有3个等位基因,干扰素α1有IFNαD、IFNα1和IFNα1/158Val3个等位基因,干扰素α2有IFNα2a、IFNα2b和IFNα2c3个等位基因,但迄今尚没有关于这两种干扰素基因变异体在中国汉族人群中组成的报道,研究意在探明这两种干扰素基因变异体分别在中国汉族人群中的分布情况。通过随机抽取100例健康中国汉族人外周血白细胞基因组DNA,采用自行设计的引物通过PCR方法扩增出干扰素α1和干扰素α2基因片段,采用第一代全自动DNA测序的方法测定出扩增的干扰素α1和干扰素α2基因片段的核苷酸序列。测序分析表明,在100例中国健康人群中仅检测出IFNα1和IFNα2b基因,且所有志愿者两种基因编码区核苷酸序列均完全相同,没有检测出干扰素α1和干扰素α2基因的其他变异体。提示IFNα1和IFNα2b分别是中国汉族人干扰素a1和干扰素a2的优势等位基因,适合中国人使用。Human interferon alpha 1 and alpha 2 gene have been found to have three alleles. Interferon alpha 1 has three alleles: IFNαD, IFNα1 and IFNα1 /158Val, while interferon alpha 2 has three alleles: IFNα2a, IFNα2b and IFNα2c. But to date, there is not the research about interferon α1 and α2 allele composition in the genomic DNA from Chinese healthy Han population. The aim of this study is to investigate the distribution of these two interferon gene variants in Chinese Han population. The white blood cell genomic DNA from 100 healthy Chinese Han subjects who randomly selected was extracted. Gene fragments of interferon alpha1 and interferon alpha2 were amplified by self-designed primers using PCR method. The nucleotide sequences of amplified interferon α1 and interferon α2 gene fragments were determined by first generation full automatic DNA sequencing. Sequencing analysis showed that only IFNα1 and IFNα2b gene was detected in 100 Chinese healthy subjects, and all of the volunteers had identical nucleotide sequences in the coding region of the both interferon α subtype gene fragments. And no other variants of the interferon alpha1 and interferon alpha2 genes were detected in these Chinese subjects. This study suggested that IFN α1 and IFN α2b were the dominant alleles of interferon a1 and interferon a2 of Chinese Han people, respectively, which were suitable for Chinese people to use in clinic.

关 键 词：干扰素α1 干扰素α2 等位基因 基因测序 

分 类 号：Q78[生物学—分子生物学]