茯砖茶提取物对秀丽隐杆线虫抗氧化及寿命的影响  

Effects of Fu brick tea extract on the antioxidant and lifespan of Caenorhabditis elegans

作　　者：曾桥 张昊亮 夏飞 胡歆 樊成 段洁 毛浓武 米柱先 ZENG Qiao;ZHANG Hao-liang;XIA Fei;HU Xin;FAN Cheng;DUAN Jie;MAO Nong-wu;MI Zhu-xian(School of Food and Biological Engineering,Shaanxi University of Science&Technology,Xi'an 710021,China;Shaanxi Research Institute of Agricultural Products Processing Technology,Xi'an 710021,China;Xianyang Jingwei Fu Tea Co.,Ltd.,Xianyang 712044,China;Shaanxi Province Product Quality Supervision and Inspection Institute,Xi'an 710048,China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]陕西农产品加工技术研究院,陕西西安710021 [3]咸阳泾渭茯茶有限公司,陕西咸阳712044 [4]陕西省产品质量监督检验研究院,陕西西安710048

出　　处：《陕西科技大学学报》2019年第5期58-62,共5页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅重点研发计划项目(2018NY-093);陕西省创新能力支撑计划项目(2018TD-019);陕西省咸阳市科技计划项目(2017K02-108);陕西省大学生创新创业训练计划项目(201810060);陕西省质监局科技计划项目(2018)

摘　　要：以秀丽隐杆线虫(Caenorhabditis elegans,C.elegans)为模型,研究茯砖茶提取物抗氧化和延长寿命及可能的作用机制.分别饲喂线虫不同质量浓度(200、400、600、800、1000μg/mL)茯砖茶提取物,研究提取物对线虫急性氧化应激、寿命、抗氧化酶活力以及抗氧化基因表达水平的影响.结果表明,浓度为400μg/mL茯砖茶提取物对H2O2损伤组线虫的保护作用最好,和空白组相比平均存活率提高了23.12%.茯砖茶提取物延长了线虫寿命并提高线虫超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力,和空白组相比,饲喂浓度为400μg/mL茯砖茶提取物的线虫组平均寿命延长了19.82%,SOD和CAT酶活力分别提高了23.31%和38.29%.抗氧化基因表达水平测定结果表明,茯砖茶提取物能上调daf-16、ctl-1、sod-3、sir-2.1mRNA表达水平,下调age-1mRNA表达水平.In this paper,effects of Fu brick tea extract on the antioxidantion and lifespan of C.elegans and its possible mechanism.Different concentrations (200,400,600,800,1 000 μ g/mL) of the extracts of Fu brick tea were fed on C.elegans to investigate the effects of acute oxidative resistance,lifespan,antioxidant enzyme activity and the expressions of antioxidant-related genes.The results showed that the average survival rate of C.elegans fed with 400 μ g/mL Fu brick tea extract increased by 23.12%,the protective effect was the best.And significantly prolonged the lifespan of C.elegans by 19.82%,the activity of SOD and CAT increased by 23.31% and 38.29% compared with the blank group.The results of antioxidant-related genes expressions showed that the extract of Fu brick tea could up-regulate the expression of daf-16,ctl-1,sod-3,sir-2.1,and down-regulate the expression of age -1.

关 键 词：秀丽隐杆线虫 茯砖茶 寿命 抗氧化 

分 类 号：TS272.5[农业科学—茶叶生产加工]