Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism  

作　　者：LU Shaokun LI Ronghua GAO Tianlong WANG Chunlin ZHENG Weibing MU Changkao SONG Weiwei 

机构地区：[1]Key Laboratory of Applied Marine Biotechnology of Ministry of Education,Ningbo University,Ningbo 315211,China [2]Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture,Ningbo University,Ningbo 315211,China [3]Third Institute of Oceanography,State Oceanic Administration,Xiamen 361001,China

出　　处：《Journal of Ocean University of China》2019年第5期1158-1162,共5页中国海洋大学学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China (No. 4147 6124);the Natural Science Foundation of Zhejiang Province (No. LY17C190005);the Major Agriculture Program of Ningbo (No. 2017C110007);the Ningbo Science and Technology Project (No. 2016C10037);the Open Fund of Ningbo University (No. xkzsc1505);K C Wong Magana Fund in Ningbo University

摘　　要：In this study,the methylation-sensitive amplification polymorphism(MSAP)was used to compare the genomic DNA methylation level of muscle,gill and hepatopancreas of Portunus trituberculatus subjected to salinity 12 for 30 days to illustrate the epigenetic mechanism of osmoregulation.Thirty primers were used to analyze the difference of methylation level of different tissues.The results showed that the baseline methylation level of muscle,hepatopancreas and gill was 47.31%,22.94%and 17.69%,respectively.After exposed to low salinity stress,the methylation epiloci changed in the three tissues.Both demethylation and methylation processes occurred under low salinity stress.The methylation ratio decreased in muscle and gill but increased in hepatopancreas.These results indicated that DNA methylation is tissue-specific when P.trituberculatus responds to low salinity.In this study, the methylation-sensitive amplification polymorphism(MSAP) was used to compare the genomic DNA methylation level of muscle, gill and hepatopancreas of Portunus trituberculatus subjected to salinity 12 for 30 days to illustrate the epigenetic mechanism of osmoregulation. Thirty primers were used to analyze the difference of methylation level of different tissues. The results showed that the baseline methylation level of muscle, hepatopancreas and gill was 47.31%, 22.94% and 17.69%, respectively. After exposed to low salinity stress, the methylation epiloci changed in the three tissues. Both demethylation and methylation processes occurred under low salinity stress. The methylation ratio decreased in muscle and gill but increased in hepatopancreas. These results indicated that DNA methylation is tissue-specific when P. trituberculatus responds to low salinity.

关 键 词：Portunus TRITUBERCULATUS DNA METHYLATION methylation-sensitive AMPLIFICATION POLYMORPHISM low salinity gill muscle HEPATOPANCREAS 

分 类 号：P[天文地球]