Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes  

作　　者：Si Zhang Xi-Mian Xiao Fan Qi Peng-Cheng Ma Wei-Wei Zhang Cheng-Zhi Dai Dan-Feng Zhang Run-Hui Liu 

机构地区：[1]State Key Laboratory of Bioreactor Engineering, Key Laboratory for Ultrafine Materials of Ministry of Education, Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology

出　　处：《Chinese Journal of Polymer Science》2019年第11期1105-1112,共8页高分子科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (Nos. 21574038, 21774031, and 21861162010);the Natural Science Foundation of Shanghai (No. 18ZR1410300);the “Eastern Scholar Professorship” from Shanghai local government (No. TP2014034);the national special fund for State Key Laboratory of Bioreactor Engineering (No. 2060204);the Fundamental Research Funds for the Central Universities (No. 22221818014)

摘　　要：Gram-negative bacteria can cause serious infections and are well known problems in biomedical practices. Biofilms of gramnegative bacteria are notorious for their frequently encountered resistance toward antibiotics. We demonstrate that α/β chimeric polypeptide molecular brush (α/β CPMB) exerts potent activities against antibiotic-resistant gram-negative bacteria. MTT viability assay, bacterial colony counting, and live/dead staining all indicate that α/β CPMB not only inhibits biofilm formation of gram-negative Pseudomonas aeruginosa and Acinetobacter baumannii, but also effectively disrupts mature biofllms that are highly resistant to one of the most active antibiotics-colistin. The superior antibacterial performance of the α/β CPMB implies its potential topical applications in treating biofilms.Gram-negative bacteria can cause serious infections and are well known problems in biomedical practices.Biofilms of gramnegative bacteria are notorious for their frequently encountered resistance toward antibiotics.We demonstrate that alp chimeric polypeptide molecular brush (α/βCPMB) exerts potent activities against antibiotic-resistant gram-negative bacteria.MTT viability assay,bacterial colony counting,and live/dead staining all indicate that alp CPMB not only inhibits biofilm formation of gram-negative Pseudomonas aeruginosa and Acinetobacter baumannii,but also effectively disrupts mature biofilms that are highly resistant to one of the most active antibiotics-colistin.The superior antibacterial performance of theα/βCPMB implies its potential topical applications in treating biofilms.

关 键 词：Molecular BRUSH CHIMERIC POLYPEPTIDE BIOFILM ANTIMICROBIAL resistance Host defense peptide 

分 类 号：R91[医药卫生—药学]