巴马特色饮食模式对D-半乳糖致衰老小鼠运动能力和抗焦虑水平的影响  

Effect of Bama Characteristic Dietary Patterns on Exercise Capacity and Antianxiety Level of D-Galactose-Induced Aging Mice

作　　者：兰海静 杨伯宁[2] 黄联莉 孙荟 宋奇 黄燕婷 何潜祖[4] 李萌[4] 李全阳[1] LAN Haijing;YANG Boning;HUANG Lianli;SUN Hui;SONG Qi;HUANG Yanting;HE Qianzu;LI Meng;LI Quanyang(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;Basic Medical College,Guangxi Medical University,Nanning 530021,China;Pusheng Sanfeng of Guangxi Dairy Co. Ltd.,Nanning 530226,China;Guangxi University Hospital,Nanning 530004,China)

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]广西医科大学基础医学院,广西南宁530021 [3]广西普生三凤乳业有限公司,广西南宁530226 [4]广西大学医院,广西南宁530004

出　　处：《食品科学》2019年第19期196-203,共8页Food Science

基　　金：国家自然科学基金面上项目(31871802);南宁市重点研发计划项目(20172141)

摘　　要：为了探究新型饮食模式的抗衰老作用,本研究通过给小鼠连续9周皮下注射100 g/L D-半乳糖溶液,建立衰老模型,从第9周开始,饲喂巴马特色饮食的同时灌胃干酪乳杆菌LT-L614和发酵乳杆菌LT-P132两种益生菌,其中LT-L614和LT-P132的体积比为1∶1,活菌数为10^9 CFU/mL,灌胃量为0.2 mL/20 gmb。第18周采用旷场实验和紧绳实验检测小鼠自发探索能力、感觉运动能力、神经肌肉协调性及焦虑情况。结果表明:旷场实验中,与衰老模型组相比,巴马特色饮食模式II能显著提高衰老小鼠后肢直立数131.91%(P<0.05),小鼠跨越方格数增加19.71%(P>0.05),总活动时间显著延长至(273.72±8.78)s(P<0.05);巴马特色饮食模式Ⅱ、Ⅲ组小鼠中央区域活动时间分别显著延长73.46%和116.60%(P<0.05)。紧绳实验中,与年轻对照组相比,巴马特色饮食模式Ⅱ、Ⅲ组小鼠悬挂时间分别延长至(58.90±4.74)、(57.67±6.28)s(P>0.05),转换分值分别显著增加43.66%、39.33%(P<0.05);与衰老模型组相比,巴马特色饮食模式Ⅱ、Ⅲ组小鼠悬挂时间分别显著延长51.92%、48.75%(P<0.05),转换分值分别显著增加154.47%、146.79%(P<0.05)。结论:巴马特色饮食模式能有效改善衰老小鼠的运动协调能力及抗焦虑能力,其中巴马饮食模式Ⅱ、Ⅲ的效果最佳,能够明显逆转D-半乳糖致衰老小鼠的运动能力衰退。In order to explore the anti-aging effects of new dietary patterns, an aging mouse model was established by subcutaneous injection of 100 g/L D-galactose solution for 9 weeks, and starting from the 9 th week, the mice were fed with Bama dietary patterns and simultaneously administered orally with a mixture of two probiotics, Lactobacillus casei LT-L614 and Lactobacillus fermentans LT-P132(1:1, V/V) with a viable cell count of 109 CFU/mL at a dose of 0.2 mL/20 g body mass for another 9 weeks. The locomotor activity, sensorimotor capacity, neuromuscular coordination and anxiety status of the mice were measured by the open field test and the tight-rope test in week 18. The results showed that in the open field test, compared with the aging model group, Bama characteristic dietary pattern Ⅱ significantly increased the number of hind limb stand by 131.91%(P<0.05) and the number of squares crossed by 19.71%(P>0.05), and prolonged the total activity time to(273.72 ± 8.78) s(P<0.05). Bama characteristic dietary patterns Ⅱ and Ⅲ increased the time spent in the center of the open field by 73.46% and 116.60%(P<0.05), respectively. In the tight-rope test, compared with the control group, Bama characteristic dietary patterns Ⅱ and Ⅲ extended the suspension time up to(58.90 ± 4.74) and(57.67 ± 6.28) s(P>0.05), respectively, and the conversion score by 43.66% and 39.33%(P<0.05). Compared with the aging model group, the suspension time was prolonged by 51.92% and 48.75%(P<0.05), respectively, and the conversion score by 154.47% and 146.79%(P<0.05), respectively. Conclusion: The Bama characteristic dietary patterns can effectively improve the movement coordination ability and antianxiety ability of aging mice. Among them, dietary patterns Ⅱ and Ⅲ are the most effective, and can significantly reverse the decline of exercise capacity of D-galactose-induced aging mice.

关 键 词：巴马特色饮食模式 D-半乳糖 运动能力 抗焦虑水平 

分 类 号：R151.4[医药卫生—营养与食品卫生学]