Enantioselective Resolution of (±)-l-Phenylethyl Acetate by Using the Whole Cells of Deep-sea Bacterium Bacillus sp. DL-2  

作　　者：DONG Lu XU Yongkai ZHANG Yun SUN Aijun HU Yunfeng 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,P.R.China [2]University of Chinese Academy of Sciences,Beijing 100049,P.R.China [3]Guangdong Key Laboratory of Marine Materia Medica,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,P.R.China [4]Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,P.R.China

出　　处：《Chemical Research in Chinese Universities》2019年第5期792-798,共7页高等学校化学研究（英文版）

基　　金：Supported by the Natural Science Foundation of Guangdong Province,China(No.2018A030313151);the Senior User Project of the Research Vessel KEXUE(No.KEXUE2018G05);the Priority Research Program of the Chinese Academy of Sciences(No.XDAl 1030404);the Scientific and Technological Project of the Ocean and Fishery from Guangdong Province,China(No.A201701C12).

摘　　要：Bacillus sp. DL-2 was isolated from the deep sea of the Western Pacific and further utilized as novel biocatalysts to efficiently asymmetrically hydrolyze (±)-1-phenylethyl acetate. After the optimization of hydrolytic reactions, chiral chemicals (R)1-phenylethanol and (S)-l-phenylethyl acetate were obtained with high optical purities (96% and 99.8%, respectively). Our research is about the asymmeric hydrolysis of (±)-1-phenylethyl acetate using whole-cell biocatalysts. In addition, the optical purity of (S)-l-phenylethyl acetate generated through the kinetic resolution of (±)-1-phenylethyl acetate using the whole-cells of Bacillus sp. DL-2 was the highest report so far. Using the whole cells of deep sea bacterium Bacillus sp. DL-2 as the biocatalysts is an enviromnentally friendly method and will play critical roles in industrial asymmetric synthesis.

关 键 词：DEEP-SEA MICROORGANISM WHOLE-CELL BIOCATALYST Asymmetric hydrolysis (R)-1-Phenylethanol (S)-1-Phenylethyl acetate 

分 类 号：TP87[自动化与计算机技术—检测技术与自动化装置]