Age-related changes of senescence-accelerated mouse prone 10 (SAMP10) mice as an animal model for AD  

作　　者：Lei Yu Linna Gao Xue Bai Ruomei Che Xiaoli He 

机构地区：[1]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China [2]Hebei North University,Zhangjiakou,China [3]Key Laboratory of Molecular Pharmacology and Drug Evaluation,Ministry of Education,Yantai University,Yantai,China

出　　处：《Life Research》2019年第4期124-136,共13页TMR生命研究

基　　金：the National Natural Science Foundation of China (Grant number:No.81473586,No.81202192).

摘　　要：Background: To explore the influence of age-related changes in learning and memory capacity of SAMP10, an Alzheimer's disease (AD) model mice, and provide theoretical foundation for the selection of month age in related experiment. Methods: SAMP10 female mice with the age of 3, 6 and 9 months were used as the objects of experiment, while the age-matched female SAMR1 were used as the controls, with 12 in each group. The learning memory capacity of mice at different age was detected through Morris water maze and step-down passive avoidance test;meanwhile, the acetylcholine, acetylcholinesterase, choline acetyltransferase, and M-cholinergic receptor binding capacity levels were determined to detect the cholinergic system damage degree in mice with different month age. In addition, the contents of monoamine neurotransmitters such as dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, norepinephrine and 5-HT, as well as those of amino acid transmitters such as glutamic acid, glutamine, aspartic acid,γ-aminobutyric acid, taurine and glycine in the brain cortex were detected by high performance liquid chromatography-electrochemical deposition. Besides, changes in hippocampal neurons were observed through Nissl staining, and the changes of Aβ in hippocampal CA1 and CA2 regions of SAMP10 were also detected by immunohistochemistry so as to explore the effects of age on the memory capacity of SAMP10. Results: It was discovered in the behavior test and AD-related index tests that: there was no significant difference between the age-matched SAMR1 and the SAMP10 at the age of 3 and 6 months. But the 9-months-old mice suffered remarkable senescence characteristics, including obviously declined learning memory capacity;down-regulated neurotransmitter levels, enzyme activities and amino acid expression;reduced hippocampal neuron number;and increased deposition of hippocampal Aβ protein. Conclusion: It is discovered in this study through behavior tests and AD-related indexs detection that, the learning memory capacity

关 键 词：Senescence-accelerated MICE 10 Alzheimer's disease Learning and memory IMPAIRMENT CHOLINERGIC system Monamine Amino acid Aβ 

分 类 号：Q[生物学]