杜仲皮水提物对虹鳟肌肉生肌调节因子家族（MRFs）基因表达的影响  

Effect of water extract of Eucommia ulmoides Oliver on the expression of MRFs gene in muscle of rainbow trout

作　　者：罗学评 张安青 袁国尚 姜海波[1,2,4] 文明[1,4] Luo Xueping;Zhang Anqing;Yuan Guoshang;Jiang Haibo;Wen Ming

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025 [3]铜仁市农业农村局渔业技术推广站,贵州铜仁554300 [4]贵州省动物疫病与兽医公共卫生重点实验室,贵州贵阳550025

出　　处：《饲料工业》2019年第20期10-16,共7页Feed Industry

基　　金：贵州省科技支撑计划项目[黔科合支撑[2016]2502号、[2016]2511号、[2017]2532号];贵州省科技平台及人才团队计划[黔科合平台人才（2018)5253];贵州省科技创新人才团队项目[黔科合人才团队（2015)4016号];高原山地动物遗传育种与繁殖教育部重点实验室（贵州大学）开放基金资助项目[GYSD-K-2018-04];贵州大学“本科教学工程”建设项目[JG201711]

摘　　要：为了探索杜仲皮水提物对虹鳟肌肉生肌调节因子家族(myogenic regulator factors, MRFs)的调节作用,采用荧光定量PCR法对其mRNA表达量进行检测分析。本研究选取初始体重为(145.56±4.12) g的虹鳟进行试验,设置5个处理组,即在基础饲料中分别添加质量分数为0%(对照组)、0.5%、1.0%、2.0%和4.0%杜仲皮的水提物,养殖10周。结果显示,杜仲皮水提物对虹鳟肌肉各处理组之间Myf6、MyoD和Myf5基因表达量差异不显著(P>0.05);但是对MyoG基因表达量影响较为明显,其中2.0%组肌肉MyoG基因表达量显著高于各处理组(P<0.05),4.0%组肌肉MyoG基因表达量显著高于对照组、0.5%组和1%组(P<0.05)。杜仲皮水提物可通过调控虹鳟肌肉MyoG基因的表达以实现肉质改善。本研究结果为进一步深入探究杜仲改善养殖动物肌肉品质的分子机理提供了一定的理论依据。In order to study the effect of the wa. ter extract of Eucommia ulmoides Oliver on the family of the MRFs gene in the muscle of rain. bow trout, the mRNA expression of the muscle tissue was analyzed by the fluorescence quantita. tive PCR from the molecular level. The fish with an initial body weight of (145.56±4.12) g were fed one of the five diets for 10 weeks, including 0 g/kg (control, 0%), 5.00 g/kg (0.5%), 10.00 g/kg (1.0%), 20.00 g/kg (2.0%) and 40.00 g/kg (4.0%) barks extract. The results showed that there were no significant differences in the expression of Myf6, MyoD and Myf5 genes among all the treatments (P>0.05). However, the expression of MyoG gene in 2.0% group was significantly higher than that of the other groups (P<0.05), and the expression of MyoG gene in 4.0%group was significantly higher than that of control group, 0.5% group and 1%group (P<0.05). The water extract of Eucommia ulmoides could regulate the expression of MyoG gene in muscle of rainbow trout.The results of this study provide a basic theory for further exploring the molecular mechanism of E. ulmoides Oliver in improving muscle quality of cultured animals.

关 键 词：杜仲 虹鳟 荧光定量PCR MRFs基因表达 肌肉品质 

分 类 号：S816.7[农业科学—饲料科学]