利用酸法水解豆粕小肽工艺研究  

Study on small peptide production technology of soybean meal hydrolyzed by acid

作　　者：谭敬仪 曹志勇[2] 黄伟[1] 熊思然 郑跃 余开文 陶琳丽[1,3] 杨秀娟[1,3] Tan Jingyi;Cao Zhiyong;Huang Wei;Xiong Siran;Zheng Yue;Yu Kaiwen;Tao Linli;Yang Xiujuan

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南农业大学基础与信息工程学院,云南昆明650201 [3]云南农业大学云南省动物营养与饲料重点实验室,云南昆明650201

出　　处：《饲料工业》2019年第20期40-43,共4页Feed Industry

基　　金：云南省动物营养与饲料重点实验室开放基金项目[DYKF2015007];校级大学生创新创业训练计划项目

摘　　要：研究采用豆粕制备2~3个氨基酸组成的小肽,为豆粕小肽的深加工和综合利用提供科学的方法和依据。试验采用三因素三水平的正交试验设计,在110℃温度下对盐酸浓度、水解时间、料液比对豆粕制备小肽的水解工艺条件进行研究。通过平均肽链长度和氮回收率,确定最佳的盐酸水解豆粕小肽的工艺条件。结果表明,显著分析可以得出三种因素对氨基态氮、总氮、水解度、氮回收率等指标的影响是显著的(P<0.05),通过极差分析可以得出盐酸浓度是影响游离氮、总氮、水解度、氮回收率等指标的主要因素。豆粕水解小肽水解时间为5h、盐酸浓度为3mol/l、料液比为1..5时水解度在30%~50%,达到试验目的2~3个氨基酸残基的大豆小肽,氮回收率达到79.59%。In this study, soybean meal was used to prepare small peptides consisting of 2~3 amino ac. ids, providing a scientific method and basis for further processing and comprehensive utilization of small peptides from soybean meal. The orthogonal design of three factors and three levels was used to study the hydrolysis conditions including concentration of hydrochloric acid, hydrolysis time and ratio of feed to liquid from soybean meal at 110 ℃ for small peptides preparation. The optimum hydrolysis conditions of soybean meal by hydrochloric acid were determined by average length of peptide chain and nitrogen recovery rate. The results showed that the three factors had significant effects on amino acid nitrogen, total nitrogen, hydrolysis degree and nitrogen recovery rate (P<0.05). The concentration of hydrochloric acid was the main factor affecting free nitrogen, total nitrogen, hydrolysis degree and ni. trogen recovery rate by range analysis. When the hydrolysis time of soybean meal is 5 h, the concentra. tion of hydrochloric acid is 3 mol/l and the ratio of material to liquid is 1..5, the degree of hydrolysis is 30%~50%. The small peptide with 2~3 amino acid residues from soybean meal achieves the experi. mental purpose, and the nitrogen recovery rate reaches 79.59%.

关 键 词：豆粕 小肽 盐酸 水解度 工艺条件 氨基态氮 总氮 

分 类 号：S816.7[农业科学—饲料科学]