富含巯基化合物酿酒酵母的筛选及发酵培养基优化  

Screening of High Intracellular Thiol Compounds Saccharomyces Cerevisiae and Optimization of Fermentation Medium

作　　者：褚金磊 李欣 王志 代俊 陈雄 CHU Jinlei;LI Xin;WANG Zhi;DAI Jun;CHEN Xiong(Key Laboratory of Ministry of Education for Fermentation Engineering, Hubei Provincial Cooperative Innovation Center for Industrial Fermentation , School of Biological Engineering and Food Science , Hubei Univ , of Tech., Wuhan 430068,China)

机构地区：[1]湖北工业大学生物工程与食品学院发酵工程教育部重点试验室工业发酵湖北省协同创新中心

出　　处：《湖北工业大学学报》2019年第5期32-36,共5页Journal of Hubei University of Technology

摘　　要：从实验室保藏的菌种中筛选出一株富含巯基化合物酿酒酵母YC-5,在初始培养基中胞内巯基含量最高为1.14%。采用响应面法对酿酒酵母YC-5的培养基配方进行优化,建立糖蜜、蛋白胨和(NH4)2SO4用量的二次回归模型,确定培养基最佳配方为:糖蜜,70mL/L;酵母浸粉,5g/L;蛋白胨,8g/L;MgSO4·7H2O,0.8g/L;KH2PO4,0.6g/L;(NH4)2SO4,0.2g/L。在通过响应面法优化后的最适培养基中发酵12h,胞内巯基含量最高可达1.82%,比未优化前提高了60%。strain of Saccharomyces cerevisiae YC5which was rich in the sulfhydryl was screened from the strains preserved in laboratory.The highest intracellular thiol content was 1. 14%in the initial medium. The response surface method was used to optimize the medium formula of Saccharomyces cerevisiae YC5 , and a quadratic regression model of molasses , peptone and ( NH4)2SO4 dosage was established.The opti - mal formula for determining the medium was set as follows : molasses 70mL / L , yeast extract 5g / L , peptone 8g / L , MgSO4 · 7H2O 0. 8g / L 、 KH2PO40. 6g / L 、( NH4 )2SO40.2g / L.After 12hof fermentation in the optimum medium optimized by response surface methodology , the intracellular sulfhydryl content was as high as to 1. 82%, which was 60% higher than that before optimization.

关 键 词：酿酒酵母 巯基含量测定 培养基优化 响应面法 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]