PET imaging on neurofunctional changes after optogenetic stimulation in a rat model of panic disorder  

作　　者：Xiao He Chentao Jin Mindi Ma Rui Zhou Shuang Wu Haoying Huang Yuting Li Qiaozhen Chen Mingrong Zhang Hong Zhang Mei Tian 

机构地区：[1]Department of Nuclear Medicine and Medical PET Center,The Second Hospital of Zhejiang University School of Medicine,Hangzhou 310009,China [2]Key Laboratory of Medical Molecular Imaging of Zhejiang Province,Hangzhou 310009,China [3]Institute of Nuclear Medicine and Molecular Imaging,Zhejiang University,Hangzhou 310009,China [4]Department of Psychiatry,The Second Hospital of Zhejiang University School of Medicine,Hangzhou 310009,China [5]Department of Advanced Nuclear Medicine Sciences,National Institute of Radiological Sciences,National Institutes for Quantum and Radiological Science and Technology,Anagawa 4-9-1,Inage-ku,Chiba,263- 8555,Japan

出　　处：《Frontiers of Medicine》2019年第5期602-609,共8页医学前沿（英文版）

基　　金：We thank Prof.Binggui Sun for providing devices and laboratory space in virus injection, Binbin Nie for technical support in Statistical Parametric Mapping analysis and Qianyun Liu (Hopstem Biotechnology LLC) for technical support on immunostaining.Help from the Zhejiang University Intelligence Convergence was greatly appreciated.This work was supported by grants from the National Natural Science Foundation of China (No.81425015);the National Key Research and Development Program of China (No.2016YFA0100900);the National Natural Science Foundation of China (Nos.81725009, 81761148029, and 81571711);Zhejiang University K.P.Chao’s High Technology Development Foundation.

摘　　要：Panic disorder (PD) is an acute paroxysmal anxiety disorder with poorly understood pathophysiology. The dorsal periaqueductal gray (dPAG) is involved in the genesis of PD. However, the downstream neurofunctional changes of the dPAG during panic attacks have yet to be evaluated in vivo. In this study, optogenetic stimulation to the dPAG was performed to induce panic-like behaviors, and in vivo positron emission tomography (PET) imaging with ,8F-flurodeoxyglucose (18F-FDG) was conducted to evaluate neurofunctional changes before and after the optogenetic stimulation. Compared with the baseline, post-optogenetic stimulation PET imaging demonstrated that the glucose metabolism significantly increased (P < 0.001) in dPAG, the cuneiform nucleus, the cerebellar lobule, the cingulate cortex, the alveus of the hippocampus, the primary visual cortex, the septohypothalamic nucleus, and the retrosplenial granular cortex but significantly decreased (P < 0.001) in the basal ganglia, the frontal cortex, the forceps minor corpus callosum, the primary somatosensory cortex, the primary motor cortex, the secondary visual cortex, and the dorsal lateral geniculate nucleus. Taken together, these data indicated that in vivo PET imaging can successfully detect downstream neurofunctional changes involved in the panic attacks after optogenetic stimulation to the dPAG.

关 键 词：PANIC DISORDER (PD) POSITRON emission tomography (PET) OPTOGENETICS DORSAL periaqueductal GRAY (dPAG) 

分 类 号：R[医药卫生]