酶法水解牛骨蛋白制备蛋白胨生化试剂的工艺  

Study on Enzymolysis Technologyof Making Biochemical Reagent-Peptone by Bone Protein

作　　者：黄菊 杨君丽 曾吉伟 Huang Ju;Yang Jun-li;ZengJi-wei(Nanning Pangbo Biological Engineering Co.,Ltd.,Guangxi Nannning530031)

机构地区：[1]南宁庞博生物工程有限公司

出　　处：《生物化工》2019年第5期51-54,58,共5页Biological Chemical Engineering

摘　　要：本文主要针对酶法水解牛骨蛋白的工艺进行研究。通过试验研究得出,牛骨先经115℃与0.2MPa压力下蒸煮6h,再加复合蛋白酶0.4%,控制pH6.5,55℃水解16h,所得胰蛋白胨中游离氨基酸4.329%、总氮14.86%、水解氨基酸80.55%,培养细菌OD600值比市售的胰蛋白胨高20%以上。其研究结果可为高品质骨蛋白胨的研发与应用提供帮助。The experimental research showed thatBovine bone was stewed underthe temperature of 115 ℃ the pressure of 0.2 MPa for 6 h.Then 0.4% compound protease was added under the temperature of 55 ℃,pH 6.5 for 16 h.The results showed that the content of amino nitrogen was 4.329%,the nitrogen was 14.86%, and the hydrolyzed amino acids was 80.55%,and the OD600value of bacterial culture was 20%, higher thantryptone sold in the market.The study results offered the research and application to high quality ofbone peptone.

关 键 词：牛骨 复合蛋白酶 蛋白胨 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]