3种快速检测沙门氏菌方法的比较分析  

Comparative analysis of 3 rapid detection methods for Salmonella

作　　者：蔡标[1] 戴陈伟[1] 吕涵 刁慧敏[1] 江杨帆[1] 辛及娣 CAI Biao;DAI Chen-Wei;LV Han;DIAO Hui-Min;JIANG Yang-Fan;XIN Ji-Di(The Medical Science Institute of Anhui Province,Hefei 230061,China)

机构地区：[1]安徽省医学科学研究院

出　　处：《食品安全质量检测学报》2019年第18期6036-6041,共6页Journal of Food Safety and Quality

基　　金：安徽省“十三五”医疗卫生重点专科项目(皖卫科教[2017]30号);安徽省卫生计生委2018年委课题(2018YK005)~~

摘　　要：目的比较PCR法、胶体金法和VITEK-2Compact法检测沙门氏菌的检测效果。方法使用PCR法、胶体金法和VITEK-2 Compact法和国标法分别对待测样品进行检测,并将3种快速检测方法的检测结果与国标法检测结果进行对比分析。结果3种快速检测法的检测结果和国标法检测结果符合率分别为100%、92.9%和100%,PCR法和VITEK-2 Compact法符合率略高于胶体金法,但3种快速检测方法总检出率与国标法比较差异均无统计学意义(P>0.05),PCR法、胶体金法和VITEK-2 Compact法对35个沙门氏菌阳性样本的检出率分别为100%、85.7%和100%。针对阳性样品,PCR法和VITEK-2 Compact法高于胶体金法,且差异有统计学意义(P<0.05)。PCR法和VITEK-2 Compact法对沙门氏菌的最低检出限为1×10^1CFU/mL,而胶体金法为1×10^2CFU/mL。结论3种快速检测方法均可作为沙门氏菌国标检测方法的有效补充。Objective To compare the detection results of Salmonella by the method of PCR,colloidal gold and VITEK-2 Compact,respectively.Methods The samples were tested by the method of PCR,colloidal gold and VITEK-2 Compact and national standard,and the detection results of the former 3 kinds of rapid detection methods were compared with the national standard method.Results Compared with the national standard method,the coincidence rates of PCR,colloidal gold and VITEK-2 Compact were 100%,92.9%,and 100%%,respectively.The PCR and VETIK method had the same coincidence rate,and slightly higer than the colloidal gold method,but there was no significant difference between the detection rates of the 3 rapid detection methods and the national standard method(P>0.05).The detection rates of 35 Salmonella positive samples by PCR,colloidal gold and VITEK-2 Compact were 100%,85.7%and 100%,respectively.The PCR method and VITEK-2 Compact method were higher than the colloidal gold method for the positive sample,and the difference was statistical significance(P<0.05).The minimum detection limit for Salmonella by PCR and VITEK-2 Compact was 1×10^1 CFU/mL,while the colloidal gold method was 1×10^2 CFU/mL.Conclusion All the 3 rapid detection methods can be used as an effective supplement to the Salmonella national standard.

关 键 词：沙门氏菌 快速检测 胶体金 PCR VITEK-2Compact 

分 类 号：R446.5[医药卫生—诊断学]