Elucidating the interaction of kansui and licorice by comparative plasma/tissue metabolomics and a heatmap with relative fold change  

作　　者：Yan-Yan Chen Juan Shen Yu-Ping Tang Jin-Gao Yu Jing Wang Shi-Jun Yue Jie Yang Jia-Qian Chen Li-Mei Feng Zhen-Hua Zhu Wei-Wei Tao Li Zhang Jin-Ao Duan 

机构地区：[1]Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility,State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research,and Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Shaanxi University of Chinese Medicine,Xi’an 712046,Shaanxi Province,China [2]Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,and Jiangsu Key Laboratory for High Technology Research of TCM Formulae,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China

出　　处：《Journal of Pharmaceutical Analysis》2019年第5期312-323,共12页药物分析学报（英文版）

基　　金：financially supported by the National Basic Research Program of China (2011CB505300,2011CB505303);the National Natural Science Foundation of China (81603258, 81673599,81773882);Key Research Project in Basic Science of Jiangsu College and University (14KJA360001);Youth Talent Project Funded by Shaanxi Higher Education Association for Science and Technology (20180307);333 High Level Talents Training Project Funded by Jiangsu Province (BRA2016387);financially supported by a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD);the Discipline Innovation Team Program of Shaanxi University of Chinese Medicine (2019-YL10)

摘　　要：Although compatibility is highly advocated in traditional Chinese medicine (TCM), inappropriate combination of some herbs may reduce the therapeutic action and even produce toxic effects. Kansui and licorice, one of TCM “Eighteen Incompatible Medicaments”, are the most representative cases of improper herbal combination, which may still be applied simultaneously under given conditions. However, the potential mechanism of their compatibility and incompatibility is unclear. In the present study, two different ratios of kansui and licorice, representing their compatibility and incompatibility respectively, were designed to elucidate their interaction by comparative plasma/tissue metabolomics and a heatmap with relative fold change. As a result, glycocholic acid, prostaglandin F2a, dihydroceramide and sphinganine were screened out as the principal alternative biomarkers of compatibility group;sphinganine, dihydroceramide, arachidonic acid, leukotriene B4, acetoacetic acid and linoleic acid were those of incompatibility group. Based on the values of biomarkers in each tissue, the liver was identified as the compatible target organ, while the heart, liver, and kidney were the incompatible target organs. Furthermore, important pathways for compatibility and incompatibility were also constructed. These results help us to better understand and utilize the two herbs, and the study was the first to reveal some innate characters of herbs related to TCM “Eighteen Incompatible Medicaments”.Although compatibility is highly advocated in traditional Chinese medicine (TCM),inappropriate combination of some herbs may reduce the therapeutic action and even produce toxic effects.Kansui and licorice,one of TCM "Eighteen Incompatible Medicaments",are the most representative cases of improper herbal combination,which may still be applied simultaneously under given conditions.However,the potential mechanism of their compatibility and incompatibility is unclear.In the present study,two different ratios of kansui and licorice,representing their compatibility and incompatibility respectively,were designed to elucidate their interaction by comparative plasma/tissue metabolomics and a heatmap with relative fold change.As a result,glycocholic acid,prostaglandin F2a,dihydroceramide and sphinganine were screened out as the principal alternative biomarkers of compatibility group;sphinganine.dihydroceramide,arachidonic acid,leukotriene B4,acetoacetic acid and linoleic acid were those of incompatibility group.Based on the values of biomarkers in each tissue,the liver was identified as the compatible target organ,while the heart,liver,and kidney were the incompatible target organs.Furthermore,important pathways for compatibility and incompatibility were also constructed.These results help us to better understand and utilize the two herbs,and the study was the first to reveal some innate characters of herbs related to TCM "Eighteen Incompatible Medicaments".

关 键 词：COMPARATIVE metabolomics Kansui LICORICE INCOMPATIBILITY Heatmap 

分 类 号：R[医药卫生]