β-环糊精增敏荧光法测定氟氰戊菊酯  

Determination of Flucythrinate by Sensitizing Effect of β -Cyclodextrin

作　　者：程杨[1] 聂继云[1] 刘红弟[1] 闫震[1] 匡立学[1] CHENG Yang;NIE Ji-yun;LIU Hong-di;YAN Zhen;KUANG Li-xue(Laboratory of Quality andSafety Risk Assessment for Fruit(Xingcheng),Ministry of Agriculture and Rural Affairs,Supervision and Test Center of Fruit and Nursery Stocks Quality(Xingcheng),Ministry of Agriculture and Rural Affairs,Xingcheng 125100)

机构地区：[1]中国农业科学院果树研究所农业农村部果品质量安全风险评估实验室(兴城)农业农村部果品及苗木质量监督检验测试中心(兴城)

出　　处：《分析科学学报》2019年第5期625-628,共4页Journal of Analytical Science

基　　金：中国农业科学院科技创新工程(CAAS-ASTIP);中央级公益性科研院所基本科研业务费专项;现代农业产业技术体系项目(CARS-27)

摘　　要：采用荧光光谱法研究了β-环糊精(β-CD)与氟氰戊菊酯的超分子相互作用。氟氰戊菊酯在λex=251 nm,λem=306 nm处发射荧光,经β-CD包合增敏后,荧光强度增强。探讨了pH值和β-CD浓度对于氟氰戊菊酯荧光强度的影响。研究发现,在pH=5.02时β-CD对氟氰戊菊酯增敏效果最好,β-CD和氟氰戊菊酯形成1∶1的超分子体系,根据经典双倒数法计算其包合常数K=148 L/mol。据此建立了测定氟氰戊菊酯的荧光新方法。方法的线性范围为1~30 ng/mL,相关系数为0.9975,检出限为0.25 ng/mL,相对标准偏差为1.3%。该方法快速、灵敏度高、稳定性好,已成功应用于水果样品中氟氰戊菊酯的测定。The supramolecular interaction between β-cyclodextrin(β-CD) and flucythrinate was studied by fluorescence spectroscopy in this paper. Fluorescence emitted at λex=251 nm and λem=306 nm, and the fluorescence intensity was enhanced through β-CD inclusion sensitization. The effects of pH and β-CD concentration on the fluorescence intensity of flucythrinate were discussed. The results showed that the fluorescence intensity of flucythrinate was greatly enhanced by β-CD at pH=5.02.β-CD and flucythrinate formed a 1∶1 supramolecular system, and the inclusion constant was calculated as K=148 L/mol by the classical double reciprocal method. A new fluorescence method for determination of flucythrinate was then established. The method had a linear range of 1-30 ng/mL, the correlation coefficient was 0.9975, the detection limit was 0.25 ng/mL, and the relative standard deviation was 1.3%. This method provides a theoretical basis for the fluorescence enhancement effect of β-CD as a supramolecular host compound for the guest molecule. Moreover, this method has good stability, which was successfully applied to the determination of flucythrinate in fruits.

关 键 词：Β-环糊精 氟氰戊菊酯 荧光光谱 包合作用 

分 类 号：O657.39[理学—分析化学]