辅酶Q10对D-半乳糖致雄性小鼠骨微结构和肌原纤维的影响  

Effects of coenzyme Q10 on bone microstructure and myofibril structure induced by D-galactose in male mice

作　　者：王可欣 陈彩玲 郑晓艳 刘翠娟 崔燎 许碧莲[1,2] 吴铁 WANG Ke-xin;CHEN Cai-ling;ZHENG Xiao-yan;LIU Cui-juan;CUI Liao;XU Bi-lian;WU Tie(Dept of Pharmacology, Guangdong Medical University;Key Lab of Nature Drug Research andDevelopment, Zhanjiang, Guangdong 524023,China;Guangdong Medical University & Guangdong RunheBiological Technology Co., Ltd. Coenzyme Q 10 Research Institute, Dongguan, Guangdong 523808, China)

机构地区：[1]广东医科大学药理教研室 [2]广东天然药物研究与开发重点实验室,广东湛江524023 [3]广东医科大学·广东润和生物科技有限公司辅酶Q10联合研究中心,广东东莞523808

出　　处：《中国药理学通报》2019年第11期1544-1550,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81373499)

摘　　要：目的 观察辅酶Q 10 对D-半乳糖致雄性小鼠骨微结构和肌原纤维的影响。方法 40只雄性小鼠、随机分为正常对照组、D-半乳糖模型组、骨化三醇组、辅酶Q 10 组,连续给药12周。实验结束时,取小鼠股骨用于micro-CT、骨生物力学检测,取腓肠肌进行超微透射电镜检查。结果 与正常对照组相比,模型组股骨最大载荷和刚性系数明显下降( P <0.01);micro-CT参数骨连接密度(Conn.D)和骨密度(BMD)均明显降低( P <0.05),而骨小梁分离度(Tb.Sp)明显增加( P <0.05);肌原纤维中肌小节、I带、H带、M线和Z线长度明显增加( P <0.01),重叠肌动蛋白和肌球蛋白肌丝长度明显缩短( P <0.01)。与模型组相比,辅酶Q 10 组股骨最大载荷、Conn.D.、BMD和Tb.Th均明显增加( P <0.05),而结构模型指数(SMI)和Tb.Sp明显下降( P <0.01);肌原纤维中I带、H带、M线和Z线均明显缩短( P <0.01)。结论 辅酶Q 10 能改善D-半乳糖所致骨微观结构破坏及腓肠肌收缩性能下降。Aim To observe the effects of CoQ 10 on bone microstructure and myofibril structure induced by D-galactose in male mice. Methods Forty male mice were randomly divided into control group, model group, calcitriol group and CoQ 10 group, in which drugs were given for 12 weeks. At the end of the experiment, the mice were sacrificed and the femurs were taken for micro-CT and biomechanics analysis. The gastrocnemius muscle was taken for transmission electron microscope examination. Results Compared with control group, the maximum load and stiffness of femur in model group significantly decreased ( P <0.01). The micro-CT parameters showed that Conn.D and BMD markedly decreased ( P <0.05), while Tb.Sp evidently increased ( P <0.05). In model group, the gastrocnemius muscle fibrils parameters showed that the length of sarcomere, I-band, H-zone, M-line and Z-line significantly increased ( P <0.01), while the length of overlapped actin and myosin filaments significantly decreased ( P <0.01). Compared with model group, the maximum load, Conn.D., BMD and Tb.Th of femur in coenzyme Q 10 group markedly increased ( P <0.05), while SMI and Tb.Sp significantly decreased ( P <0.01). Moreover, I-band, H-zone, M-line and Z-line were significantly shortened ( P <0.01). Conclusion Coenzyme Q 10 can improve bone microstructure damage and the contractility of gastrocnemius muscle in male mice induced by D-galactose.

关 键 词：骨质疏松症 肌少症 辅酶Q 10 D-半乳糖 骨微结构 肌原纤维 

分 类 号：R-332[医药卫生] R322.74