蕨菜超临界萃取物化学成分及其抑制3T3-L1前脂肪细胞分化作用研究  

Chemical constituents of Pteridium aquilinum supercritical extract and its anti-adipogenic effect on 3T3-L1 preadipocytes

作　　者：吴永祥[1,2] 张瑶 卢玮玮 胡晓倩 權泰亨[2] 金泰完 WU Yong-xiang;ZHANG Yao;LU Wei-wei;HU Xiao-qian;KWON Tae-hyung;KIM Tae-wan(College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041, China;Dept of Food Science and Biotechnology, Andong National University, Andong 760749, Korea)

机构地区：[1]黄山学院生命与环境科学学院,安徽黄山245041 [2]安东国立大学食品科学与生物技术学院,韩国安东760749

出　　处：《中国药理学通报》2019年第11期1590-1595,共6页Chinese Pharmacological Bulletin

基　　金：安徽省高校优秀拔尖人才培育项目(No gxgnfx2019037);安徽省留学回国人员创新项目择优资助计划重点项目(No 2017srst1);博士人才引进启动项目(No 2016xkjq004)

摘　　要：目的 分析蕨菜超临界CO 2萃取物(SFE-PA)的化学成分,并探究萃取物对3T3-L1前脂肪细胞分化的抑制作用及机制。方法 采用GC-MS分析SFE-PA主要化学成分。体外培养3T3-L1前脂肪细胞,构建脂肪细胞分化的细胞模型,MTT法测定细胞毒性,采用油红O染色法分析SFE-PA对脂肪细胞分化的抑制作用,利用qRT-PCR检测脂肪细胞分化中相关基因的mRNA表达水平。结果 从SFE-PA中共鉴定出10种化合物,主要成分有4,4,5,7,8-五甲基-二氢香豆素(36.07%)、β-谷甾醇(25.66%)、4-氨基-7-二乙氨基-香豆素(8.26%)、棕榈酸(5.05%)等。SFE-PA浓度依赖性地抑制3T3-L1前脂肪细胞分化,并减少细胞中脂质的沉积。同时,SFE-PA明显下调PPARγ、CEBPα、SREBP-1c、FAS、ACC的mRNA表达水平。结论 SFE-PA具有明显抑制3T3-L1前脂肪细胞分化的作用,这一作用与其调控脂肪细胞分化转录调控因子和脂质合成相关基因等密切相关,而高含量的酚类、醇类化合物可能是其发挥作用的物质基础。Aim To identify chemical constituents of supercritical extract from Pteridium aquilinum (Linn.) Kuhn (SFE-PA) and investigate its anti-adipogenic effect and underlying mechanism. Methods The chemical constituents of SFE-PA were identified by GC-MS. The differentiation of 3T3-L1 preadipocytes was induced to establish cell model. MTT assay was employed to detect cell viability. Oil Red O staining was used to analyze the effect of SFE-PA on the differentiation of 3T3-L1 preadipocytes. qRT-PCR was performed to examine the expression of genes related with adipogenesis and lipogenesis. Results Ten compounds of SFE-PA were identified. The major constituents were 4,4,5,7,8-pentamethyl-dihydrocoumarin (36.07%),β-sitosterol (25.66%), 4-amino-7-diethylamino-chromen-2-one (8.26%) and palmitic acid (5.05%). SFE-PA suppressed 3T3-L1 adipocyte differentiation and reduced fat accumulation in a dose-dependent manner. Treatment of SFE-PA significantly down-regulated the mRNA expression of PPARγ, C/EBPα, SREBP-1c, as well as FAS and ACC. Conclusions SFE-PA can inhibit the adipogenesis and differentiation of 3T3-L1 cells, and its mechanism may be closed related to its inhibition of genes expression regulating adipogenesis and lipogenesis. Phenols and alcohols might be potential bioactive components.

关 键 词：蕨菜 超临界CO 2萃取 化学成分 3T3-L1细胞 脂肪细胞分化 分子机制 

分 类 号：R282.71[医药卫生—中药学] R284.2[医药卫生—中医学]