Protective effect of ascorbic acid on cyclophosphamide induced testicular gametogenic and androgenic disorders in male rats  

作　　者：Ujjal Baran Das Mousumi Mallick Jogendra Mohan Debnath Debidas Ghosh 

机构地区：[1]Reproductive Endocrinology and Family Welfare Research Unit,Department of Human Physiology with Community Health,Vidyasagar University,Midnapore-721102,West Bengal,India.

出　　处：《Asian Journal of Andrology》2002年第3期201-207,共7页亚洲男性学杂志(英文版)

基　　金：The authors gratefully acknowledge the financial assistance from the Major Research Project(Project No.F-3/50/99 dated 3l-3-99);provided by the University Grants Commission(UGC),New Delhi,India

摘　　要：Aim:To study the detrimental effects of cyclophosphamide on the testicular androgenic and gametogenic activities through endocrine inhibition and/or induction of oxidative stress in male albino rats and to evaluate the protective effect of ascorbic acid.Methods:The testicular△^(5),3β-hydroxysteroid dehydrogenase(HSD),17β-HSD,peroxidase and catalase activities along with the levels of malondialdehyde(MDA)and conjugated dienes in testicular tissue were measured for the evaluation of testicular oxidative stress.The plasma testosterone(T)level was measured by immunoassay.Various germ cells at stageⅦof spermatogenic cycle were quantified from testicular stained sections.Results:Cyclophosphamide treatment results in a significant inhibition in the testicular△^(5),3β-HSD and 17β-HSD activities,a decrease in plasma T level and a diminution in the counts of various germ cells.Moreover,this treatment was also associated with a significant inhibition of the peroxidase and catalase activities along with high levels of MDA and conjugated dienes in the testis.All these changes were reversed by ascorbic acid co-administration.Conclusion:Cyclophosphamide treatment at the dosage used caused testicular gametogenic and androgenic disorders as well as induced testicular oxidative stress that can be reversed by ascorbic acid co-administration.

关 键 词：CYCLOPHOSPHAMIDE ANDROGENESIS GAMETOGENESIS oxidative stress free radicals ascorbic acid CATALASE PEROXIDASE testosterone 

分 类 号：R341[医药卫生—基础医学]