人肝癌细胞体外氨基酸代谢  

Metabolism of Animo Acid in Human Liver Cancer Cell Line in Vitro

作　　者：陆伟[1] 钱绍诚[1] 王凤梅[1] 孙金英[1] 阚志超[1] 李隽[1] 

机构地区：[1]天津市第三中心医院,300170

出　　处：《天津医药》2002年第11期643-645,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金项目 (项目编号 :34948)

摘　　要：目的 :研究肝癌细胞对氨基酸的代谢作用。方法 :对人肝癌细胞系 (SMMC -7721)体外培养 ,观察培养液氨基酸成分的动态变化 ,同时对人正常肝细胞做对比研究。结果 :肝癌细胞体外培养24h ,培养液中精氨酸、苏氨酸、谷氨酰胺(P<0.01)、牛磺酸(P<0.05)的含量明显下降 ;培养48h ,除上述4种氨基酸外 ,培养液中丝氨酸、半胱氨酸的含量也明显下降(P<0.05)。人正常肝细胞培养24h ,培养液中谷氨酰胺、精氨酸(P<0.01)、半胱氨酸(P<0.05)含量明显下降 ;培养48h ,培养液中谷氨酰胺、半胱氨酸、精氨酸、组氨酸(P<0.01)、丝氨酸(P<0.05)含量明显下降。结论 :肝癌细胞体外增殖过程对精氨酸、谷氨酰胺、苏氨酸、牛磺酸的消耗明显增加。此外 ,对丝氨酸、半胱氨酸也有一定的消耗 ,这6种氨基酸可能为肝癌代谢所必需 。Objective:To study the metabolism of animo acid in human liver cancer cell line in vitro.Methods:The human liver cancer cell line(SMMC-7721)and human norm al liver cells were cultured,and their changes of animo acid compositions in cul tured medium were observed respectively.Results:In SMMC-7721,the concentrations of arginine,glutamine,threomine(P<0.01)and taurin(P<0.05)significantly decrease d after 24 h;the concentrations of serine and cystein also significantly decreas ed after 48 h(P<0.05).In human normal liver cells,the concentrations of arginine ,glutamine(P<0.01)and cystein(P<0.05)significantly decreased after 24 h;the conc entrations of arginine,glutamin,cystein,histidine(P<0.01)and serine(P<0.05)signi ficantly decreased after 48 h.Conclusion:During the period of proliferation of l iver cancer cells in vitro,the comsumptions of arginine,glutamine,threomine,taur in,cystein and serine may decrease.These may be the necessary animo acid to the liver cancer cells.

关 键 词：肝癌 氨基酸代谢 细胞培养 牛磺酸 苏氨酸 

分 类 号：R735.7[医药卫生—肿瘤] R730.23[医药卫生—临床医学]