草莓果实成熟衰老与Ca^(2+)、CaM、Ca^(2+)-ATPase和活性氧代谢的关系  

Relationship Between Maturation, Senescence and Ca^(2+),CaM Content, Ca^(2+)-ATPase Activity, and Active Oxygen Metabolism in Strawberry Fruits

作　　者：关军锋[1] 高敏[1] 樊秀彩[1] 顾采琴[1] 李广敏[1] 张继澍[2] 

机构地区：[1]河北省农林科学院农业物理生理生化研究所,石家庄050051 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《中国农业科学》2002年第11期1385-1389,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 (3 0 2 70 93 3 );河北省农林科学院博士资金资助项目

摘　　要：研究了春星草莓果实成熟衰老过程中Ca2 + 、CaM含量、Ca2 + ATPase活性和活性氧代谢的变化 ,结果表明 ,随果实成熟衰老 ,可溶性Ca2 + 含量和SOD活性呈下降趋势 ,O2-·产生速率呈升高趋势 ,Ca2 + ATPase活性先达到高峰后降低。CaM含量以采前白熟期和采后明显衰老期为最高。与常温 (2 5℃ )贮藏相比 ,低温 (4℃ )贮藏中上述生化变化较为缓慢。表明细胞内钙信使系统的活化和活性氧自由基的积累与果实成熟衰老密切相关。Changes of the content of Ca 2+ and CaM, Ca 2+ ATPase activity and active oxygen metabolism during strawberry (cv. Chunxing) fruits maturation and senescence were investigated. The results showed that the soluble Ca 2+ content and SOD activity in fruits decreased and O 2 -· production rate increased, the Ca 2+ ATPase activity peaked at first and then declined during fruits maturation and senescence. There were highest CaM content at white stage in preharvest fruits and at marked senescence stage in postharvest ones. The above biochemical changes in fruits stored at low temperature(4℃)were slower than those stored at normal temperature(25℃). Thus, it indicated that the stimulation of calcium messenger system and accumulation of active oxygen free radical were closely related to fruits maturation and senescence.

关 键 词：草莓 果实成熟衰老 活性氧代谢 钙信使系统 活性氧自由基 钙离子 钙调素 

分 类 号：S668.4[农业科学—果树学] Q945.65[农业科学—园艺学]