SCL基因反义寡核苷酸对K562和CEM细胞系的作用  

Effects of SCL Antisense Oligonucleotides on K562 and CEM Cell Lines

作　　者：郑正津[1] 胡建达[1] 黄淑桦[1] 王少元[1] 吕联煌[1] 

机构地区：[1]福建医科大学附属协和医院,福建省血液病研究所福州350001

出　　处：《中国实验血液学杂志》2002年第5期404-408,共5页Journal of Experimental Hematology

基　　金：卫生部优秀青年科技人才专项科研基金资助项目编号982 9

摘　　要：干细胞白血病 (SCL)基因是新发现的与白血病发生有关的癌基因。为进一步探讨SCL反义寡核苷酸对白血病细胞的作用 ,本研究应用SCL基因的反义硫代磷酸寡脱氧核苷酸 (AS PS ODN)分别与K562和CEM白血病细胞系作用 ,观察细胞生长、分化、凋亡和SCLmRNA变化。结果显示 :(1 )AS PS ODN对K562和CEM细胞的生长有不同程度的抑制作用 ,并呈量效和时效关系 ;(2 )AS PS ODN作用后K562细胞联苯胺染色阳性率明显增加 ;(3)AS PS ODN作用后K562细胞发生凋亡现象 ,但未观察到CEM细胞凋亡 ;(4)AS PS ODN作用后K562细胞和CEM细胞SCLmRNA表达水平减低 ,同时CEM细胞SIL SCLmRNA表达水平也减低。结论 :AS PS ODN能特异性抑制K562和CEM细胞的增殖 ,减低SCLmRNA和SIL SCLmRNA表达水平 ,同时促进红系分化 。The stem cell leukemia(SCL) gene is a new oncogene related with leukemogenesis. To explore the effects of antisense oligonucleotides of SCL on leukemic cells, SCL antisense phosphorothioate oligodeoxynucleotides(AS PS ODN) were used to treat K562 and CEM leukemic cell lines to observe the effects on proliferation, differentiation, apoptosis and SCL mRNA expression in the cells. The results showed that incubation of K562 or CEM cells with AS PS ODN at different concentrations led to inhibition of cell proliferation, and the inhibitory effects varied with the incubation time. The positive rate of benzidine staining in K562 cells increased significantly after the inhibition with AS PS ODN, compared with S PS ODN treatment. The charateristics of apoptosis were observed in K562 cells treated with AS PS ODN, but not in CEM cells. Expression of SCL mRNA in K562 and CEM cells and SIL SCL mRNA in CEM cells decreased after incubation of AS PS ODN. It is concluded that SCL AS PS ODN inhibits specifically the proliferation of K562 and CEM cells, also decreases the level of SCL and SIL SCL mRNA expression. AS PS ODN enhances erythroid differentiation and induces premature apoptosis in K562 cells.

关 键 词：干细胞白血病基因 反义寡核苷酸 K562细胞系 CEM细胞系 SCL 表达水平 基因治疗 

分 类 号：R733.7[医药卫生—肿瘤] R394.[医药卫生—临床医学]