载三氧化二砷PLGA微球的制备与体外释放对肝癌的影响  

Study on Sustained Release of Arsenic Trioxide Loaded PLGA Microspheres

作　　者：吴育民[1] 杜端明[1] 陈娟萍[1] 刘春霖[1] WU Yumin;DU Duanming;CHEN Juanping(Shenzhen Second People's Hospital,Shenzhen,518035)

机构地区：[1]深圳市第二人民医院

出　　处：《实用癌症杂志》2019年第8期1223-1225,1228,共4页The Practical Journal of Cancer

基　　金：深圳市知识创新计划项目(编号:JCYJ20150330102720120)

摘　　要：目的制备载三氧化二砷(As2O3)的PLGA微球,在体外环境下模拟体内环境进行药物的缓释研究。方法采用复乳溶剂蒸发法分别制备载As2O3与空载的PLGA微球,利用恒温摇床进行体外释放,通过连续取样测定释放量,绘制释放曲线。结果经过优化配比方案,成功制备出载As2O3以及空载的PLGA微球,体外药物缓释经过连续的取样与测定绘制出释放曲线。结论制备的载As2O3以及空载的PLGA微球为粉末状固体,通过进一步的电镜表征和体外药物释放表明在体外环境下能够很好的进行药物释放。Objective To prepare As2O3 loaded PLGA microspheres and study the drug release in vitro.Methods As2O3 and no-load PLGA microspheres were prepared by double emulsion solvent evaporation method,and released in vitro by using a constant temperature rocker.The release amount was determined by continuous sampling and the release curve was drawn.Results As2O3 and no-load PLGA microspheres were successfully prepared by optimizing the formula.The drug release curve was drawn by continuous sampling and determination in vitro.Conclusion The prepared As2O3 and no-loaded PLGA microspheres are powder solid.The results of electron microscopy and in vitro drug release show that the drug release can be well carried out in vitro.

关 键 词：PLGA AS2O3 扫描电镜 缓释 释放曲线 

分 类 号：R735.7[医药卫生—肿瘤]