Bioinformatics and Expression Analysis of CaERF Gene in Capsicum  

作　　者：Jiaqi GENG Jieyu YANG Peiqi HU Ziqi ZHAO Kunpeng LI Jingying WANG Chunyu ZHANG Qingxun GUO 

机构地区：[1]College of Plant Science, Jilin University

出　　处：《Agricultural Biotechnology》2019年第4期26-28,共3页农业生物技术（英文版）

基　　金：Supported by College Students’Innovation Fund of Jilin University(2017A82372)

摘　　要：AP2/ERF transcription factor is a kind of plant-specific transcription factor,which is widely involved in the whole process of plant growth and development,and has important regulatory effects in plant secondary metabolism.In this study,the CaERF gene was cloned from Capsicum annuum by RT-PCR.The bioinformatics and expression analysis revealed that the CDS region of this gene is 795 bp in length,encoding 264 amino acids,and the molecular weight and isoelectric point of the protein are 30.1 KD and 5.74,respectively;the gene encodes a 58-amino acid DNA-binding domain AP2 at 533-706,which is closest to Capsicum chinense in genetics;and the gene is expressed early in the fruit development from 16 to 20 d after flowering,and related to the level of pun 1 gene expression.The cloning and expression analysis of CaERF transcription factor gene laid a foundation for further study on the regulation of capsaicin synthesis.AP2/ERF transcription factor is a kind of plant-specific transcription factor, which is widely involved in the whole process of plant growth and development, and has important regulatory effects in plant secondary metabolism. In this study, the CaERF gene was cloned from Capsicum annuum by RT-PCR. The bioinformatics and expression analysis revealed that the CDS region of this gene is 795 bp in length, encoding 264 amino acids, and the molecular weight and isoelectric point of the protein are 30.1 KD and 5.74, respectively; the gene encodes a 58-amino acid DNA-binding domain AP2 at 533-706, which is closest to Capsicum chinense in genetics; and the gene is expressed early in the fruit development from 16 to 20 d after flowering, and related to the level of pun1 gene expression. The cloning and expression analysis of CaERF transcription factor gene laid a foundation for further study on the regulation of capsaicin synthesis.

关 键 词：CAPSAICIN CaERF GENE GENE CLONING mRNA expression 

分 类 号：S64[农业科学—蔬菜学]