菜用黄麻粗多糖含量的测定及其单糖组成分析  

Determination of Crude Polysaccharide Content of Vegetable Jute and Analysis of Its Monosaccharide Composition

作　　者：练冬梅 姚运法 赖正锋 林碧珍 洪建基 LIAN Dong-mei;YAO Yun-fa;LAI Zheng-feng;LIN Bi-zhen;HONG Jian-ji(Institute of Subtropical Agriculture,Fujian Academy of Agricultural Sciences,Zhangzhou,Fujian 363005,China)

机构地区：[1]福建省农业科学院亚热带农业研究所

出　　处：《福建农业科技》2019年第6期14-17,共4页Fujian Agricultural Science and Technology

基　　金：农业部现代麻类产业技术体系“漳州黄/红麻试验站”(CARS-16S07);福建省农业科学院一般项目(A2017-8)

摘　　要：采用醇沉提取蒽酮硫酸法测定菜用黄麻品种闽麻菜1号粗多糖含量,以及1苯基3甲基5吡唑啉酮柱前衍生高效液相色谱法测定粗多糖的单糖组成。结果表明:菜用黄麻粗多糖含量为2.5%,主要由鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖6种单糖组成,物质的量的比为1∶0.82∶0.36∶0.12∶0.47∶0.11,其中鼠李糖含量最高,为39 968.8 mg·kg-1,葡萄糖醛酸含量次之。菜用黄麻嫩茎叶富含粗多糖及其单糖组分鼠李糖,因此菜用黄麻可作为提取多糖和鼠李糖的原料。The content of crude polysaccharide in Minmacai No.1,a variety of vegetable jute,was determined by anthrone-sulfuric acid method extracted by alcohol precipitation,and the monosaccharide composition of crude polysaccharide was determined by HPLC with pre-column derivatization of 1-phenyl-3-methyl-5-pyrazolone.The results showed that the content of crude polysaccharides in vegetable jute was 2.5%,which was mainly composed of 6 monosaccharides,namely rhamnose,glucuronic acid,galacturonic acid,glucose,galactose and arabinose.The ratio of their content was 1∶0.82∶0.36∶0.12∶0.47∶0.11,and the content of rhamnose was the highest,reaching 39 968.8 mg·kg-1,followed by the content of glucuronic acid.The young stems and leaves of vegetable jute were rich in crude polysaccharides and rhamnose,so vegetable jute could be used as the raw materials for extracting polysaccharide and rhamnose.

关 键 词：菜用黄麻 粗多糖 含量 单糖 高效液相色谱 

分 类 号：R28[医药卫生—中药学]