酶法提取苦丁茶熊果酸的工艺优化及其对CCl4致小鼠肝损伤的保护作用  

Optimization of Enzymolysis Extraction of Ursolic Acid from Ilex kudingcha C.J.Tseng and Its Protective Effects of CCl4-induced Liver Injury in Mice

作　　者：张海全[1] 钟晓坤 黄勤英[1] 农克良[1] ZHANG Hai-quan;ZHONG Xiao-kun;HUANG Qin-ying;NONG Ke-liang(Guangxi Colleges and Universities Key Laboratory Breeding Base of Chemistry of Guangxi Southwest Plant Resources,Guangxi Normal University for Nationalities,Chongzuo 532200,China)

机构地区：[1]广西民族师范学院广西高校桂西南特色植物资源化学重点实验室

出　　处：《食品工业科技》2019年第17期161-166,共6页Science and Technology of Food Industry

基　　金：广西民族师范学院科研经费资助项目(2016JX001);广西教育厅广西高校中青年教师基础能力提升项目资助(KY2016YB517)

摘　　要：为优化酶解提取苦丁茶中熊果酸的工艺,并对熊果酸的保肝作用进行研究,通过响应面分析法,研究了酶解温度、酶解pH、酶解时间对熊果酸含量的影响。以0.1%CCl4橄榄油溶液20 mL/kg腹腔注射制备小鼠肝损伤模型,检测血清中谷草转氨酶(Aspartate transaminase,AST)、谷丙转氨酶(Alanine aminotransferase,ALT)水平以及肝组织超氧化物歧化酶(Superoxide Dismutase,SOD)、丙二醛(Malondialdehyde,MDA)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)水平,并观察肝组织病理形态变化,评价苦丁茶熊果酸的保肝作用。结果表明,熊果酸的适宜提取条件为:酶解温度为48℃,酶解pH为5.0,酶解时间为1.0 h,该条件下熊果酸含量为(37.28±0.89)mg/g,该模型预测性好、与实际情况拟合较好。保肝作用研究表明,苦丁茶熊果酸能显著降低CCl4致肝损伤小鼠血清ALT和AST活性及肝组织中MDA水平,有效提高肝组织SOD和GSH-Px水平。肝组织病理学观察结果表明,苦丁茶熊果酸可以减轻CCl4对肝脏的病理损伤。本研究表明苦丁茶熊果酸对CCl4致小鼠肝损伤具有明显保护作用。In order to optimize the enzymolysis extraction process of ursolicacid(UA)from Ilex kudingcha C.J.Tseng and study on its protective effect of liver injury in mice.To research the effects on yield of UA,enzymolysis temperature,enzymolysis time and enzymolysis pH were optimized by response surface analysis.0.1%CCl4(20 mL/kg intraperitoneal injection)induced liver injury model was established in mice,then the liver protection of UA from Ilex kudingcha C.J.Tseng was evaluated by the level of AST,ALT in serum,SOD,MDA and GSH-Px in liver tissue.Histopathological examination of liver tissue also been observed.The results demonstrated that the optimum extraction condition of UA were as follows:enzymolysis temperature of 48℃,enzymolysis pH of 5.0,enzymolysis time of 1.0 h,the content of UA was(37.28±0.89)mg/g on this condition and the models provided a better fit to the real data and good predictability.The liver protection experiments showed that the UA from Ilex kudingcha C.J.Tseng could decrease the level of AST,ALT in serum,MDA in liver tissue significantly and improve the level of SOD,GSH-Px in liver tissue obviously.Liver tissue damage induced by CCl4 was ameliorated according to the histopathological examination.In conclusion,the UA from Ilex kudingcha C.J.Tseng showed hepatoprotective effects on CCl4-induced liver injury in mice.

关 键 词：苦丁茶 响应面法 熊果酸 酶解 

分 类 号：TS201.4[轻工技术与工程—食品科学]