Printing amphotericin B on microneedles using matrixassisted pulsed laser evaporation  

作　　者：Roger Sachan Panupong Jaipan Jennifer Y.Zhang Simone Degan Detlev Erdmann Jonathan Tedesco Lyndsi Vanderwal Shane J.Stafslien Irina Negut Anita Visan Gabriela Dorcioman Gabriel Socol Rodica Cristescu Douglas B.Chrisey Roger J.Narayan 

机构地区：[1]Wake Early College of Health and Sciences,Raleigh,North Carolina,USA [2]Joint Department of Biomedical Engineering,The University of North Carolina and North Carolina State University,Raleigh,North Carolina,USA [3]Department of Dermatology,Duke University Medical Center,Durham,North Carolina,USA [4]Department of Surgery,Division of Plastic,Reconstructive,Maxillofacial and Oral Surgery,Duke University Medical Center,Durham,North Carolina,USA [5]Keyence Corporation of America,Elmwood Park,New Jersey,USA [6]Office of Research and Creativity Activity,North Dakota State University,1715 Research Park Drive,Fargo ND,USA [7]National Institute for Lasers,Plasma and Radiation Physics,Lasers Department,P.O.Box MG-36,Bucharest-Magurele,Romania [8]Department of Physics and Engineering Physics,Tulane University,New Orleans,LA,USA

出　　处：《International Journal of Bioprinting》2017年第2期49-59,共11页国际生物打印期刊

基　　金：We would like to acknowledge C Mooney(NCSU Analytical Instrumentation Facility)for his aid with electron microscopy,B Andersen for her aid with Fourier transform infared spectrosocpy(NCSU College of Textiles),P Strader(NCSU Analytical Instrumentation Facility)for his aid with nanoindentation;the US National Institutes of Health(Award#1R21AI117748-01A1);the US National Science Foundation(Award CMMI 1258536);and the US Office of Naval Research(Award#N00014-15-1-2323);This work was also supported by the National Program PN 4N/2016(1647-LAPLASⅣand a grant of Ministry of Research and Innovation,CNCS–UEFISCDI,Project Number PNIII-P4-ID-PCE-2016-0884,within PNCDIⅢ.

摘　　要：Transdermal delivery of amphotericin B,a pharmacological agent with activity against fungi and parasitic protozoa,is a challenge since amphotericin B exhibits poor solubility in aqueous solutions at physiologic pH values.In this study,we have used a laser-based printing approach known as matrix-assisted pulsed laser evaporation to print amphotericin B on the surfaces of polyglycolic acid microneedles that were prepared using a combination of injection molding and drawing lithography.In a modified agar disk diffusion assay,the amphotericin B-loaded microneedles showed concentrationdependent activity against the yeast Candida albicans.The results of this study suggest that matrix-assisted pulsed laser evaporation may be used to print amphotericin B and other drugs that have complex solubility issues on the surfaces of microneedles.

关 键 词：matrix-assisted pulsed laser EVAPORATION MICRONEEDLE AMPHOTERICIN B ANTIFUNGAL 

分 类 号：TN2[电子电信—物理电子学]