BTG1过表达对胰腺癌细胞增殖、侵袭和cyclin D1、cyclin B1、MMP-2、MMP-9蛋白表达的影响  

Effects of BTG1 overexpression on proliferation, invasion and protein expression of cyclin D1, cyclin B1, MMP-2 and MMP-9 in pancreatic cancer cells

作　　者：王开琼[1] 邢贻雷[1] 乔欣 李仕总 宫东伟 余智威 吴奕强[1] WANG Kaiqiong;XING Yilei;QIAO Xin;LI Shizong;GONG Dongwei;YU Zhiwei;WU Yiqiang(Department of Biliary and Pancreatic Surgery,Hainan General Hospital,Haikou 570311,China)

机构地区：[1]海南省人民医院胆胰外科

出　　处：《中国比较医学杂志》2019年第9期81-86,共6页Chinese Journal of Comparative Medicine

摘　　要：目的探讨B细胞易位基因1(BTG1)过表达对胰腺癌细胞增殖和侵袭的影响及其机制。方法采用qRT-PCR和Western blot检测人胰腺癌细胞株PANC-1、AsPC-1、BxPc-3和正常胰腺导管上皮细胞株H6C7中BTG1 mRNA和蛋白的表达水平。将体外培养的BxPc-3细胞分为对照组(未转染)、pcDNA3.1组(转染pcDNA3.1空载体质粒)和BTG1组(转染pcDNA3.1-BTG1过表达质粒),转染48 h后,qRT-PCR和Western blot检测转染效果;MTT法、流式细胞术和Transwell小室法分别检测各组细胞的增殖能力、周期分布和侵袭能力;Western blot检测各组细胞中细胞周期蛋白D1(cyclin D1)、细胞周期蛋白B1(cyclin B1)、基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)蛋白的表达情况。结果与H6C7细胞相比,PANC-1、AsPC-1、BxPc-3细胞中BTG1 mRNA和蛋白的表达水平均明显降低(P<0.05),且BxPc-3细胞差异最为显著。与对照组相比,BTG1组细胞的增殖、侵袭能力、细胞在S期的百分比以及细胞中cyclin D1、cyclin B1、MMP-2、MMP-9蛋白的表达水平均明显降低,而细胞在G0/G1期的百分比明显升高(P<0.05);而pcDNA3.1组和对照组细胞的增殖能力、侵袭能力、周期分布情况以及cyclin D1、cyclin B1、MMP-2、MMP-9蛋白的表达水平均无统计学差异(P>0.05)。结论BTG1过表达可抑制胰腺癌细胞的增殖和侵袭,其作用机制可能与下调cyclin D1、cyclin B1、MMP-2、MMP-9蛋白的表达有关。Objective To investigate the effect of B cell translocation gene 1(BTG1)overexpression on proliferation and invasion of pancreatic cancer cells and to elucidate the mechanism.Methods The expression of BTG1 mRNA and protein in the human pancreatic cancer cell lines,PANC-1,AsPC-1 and BxPc-3,and in the normal pancreatic ductal epithelial cell line,H6C7,was determined by qRT-PCR and Western blotting,respectively.BxPc-3 cells cultured in vitro were divided into a control group(untransfected),pcDNA3.1 group(transfected with an empty pcDNA3.1 vector)and BTG1 group(transfected with pcDNA3.1-BTG1 overexpression plasmid).At 48 h after transfection,the transfection effects were examined by qRT-PCR and Western blotting.Cell proliferation,cell cycle distribution and invasion ability in each group were measured by MTT assay,flow cytometry and Transwell chamber,respectively.The expression of cell cycle protein D1(cyclin D1),cyclin B1,matrix metalloproteinase 2(MMP-2)and MMP-9 was determined by Western blotting.Results Compared with the H6C7 cells,the expression levels of BTG1 mRNA and proteins in PANC-1,AsPC-1 and BxPc-3 cells were significantly decreased(P<0.05),and the most significant difference was observed in the BxPc-3 cells.Compared with the control group,the proliferation,invasiveness,the percentage of cells in S phase and the protein expression of cyclin D1,cyclin B1,MMP-2 and MMP-9 in the BTG1 group were significantly decreased,while the percentage of cells in G0/G1 phase was significantly increased(P<0.05).There was no significant difference between the pcDNA3.1 group and the control group in cell proliferation,invasiveness,cell cycle distribution and expression of the abovementioned proteins(P>0.05).Conclusions BTG1 overexpression inhibits the proliferation and invasion of pancreatic cancer cells,and its mechanism may be associated with downregulation of cyclin D1,cyclin B1,MMP-2 and MMP-9 protein expression.

关 键 词：胰腺癌 BTG1 细胞增殖 侵袭 CYCLIN D1 MMP-9 

分 类 号：R-33[医药卫生]