抗坏血酸葡萄糖苷/β-环糊精包合物的制备与表征  

Preparation and Characterization of 2-O-α-D-glucopyranosyl-L-ascorbic Acid Inclusion Compound

作　　者：成银雏 翁婧媛 朱瀛奎 李艳[1] 贾红华[1] CHENG Yin-chu;WENG Jing-yuan;ZHU Yin-kui;LI Yan;JIA Hong-hua(College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 211800,China)

机构地区：[1]南京工业大学生物与制药工程学院

出　　处：《现代食品科技》2019年第9期204-209,257,共7页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(21878155);江苏省先进生物制造协同创新中心项目;江苏省品牌专业建设工程项目;江苏省优势学科建设工程项目;江苏高校“青蓝工程”计划项目;江苏省“六大人才高峰”高层次人才计划项目

摘　　要：本文研究了抗坏血酸葡萄糖苷(2-O-α-D-glucopyranosyl-L-ascorbic acid,简称AA-2G)/β-环糊精包合物的制备工艺,以提高它在应用中的稳定性、生物利用度。选用β-环糊精(β-cyclodextrin,β-CD)对AA-2G进行包合,采用饱和水溶液法研究了AA-2G-β-CD包合物的制备工艺。以包合率为考察指标,通过单因素试验考察了温度、时间、搅拌速度以及β-环糊精和AA-2G的摩尔比对包合物制备效果的影响。进一步运用正交试验研究确定了AA-2G-β-CD包合物的最佳工艺条件为:AA-2G与β-CD的摩尔比为1:3,温度为60℃、搅拌速度为200 r/min,时间为5 h时,包合率为49.55%。影响包合率的因素顺序为:时间>温度>转速>摩尔比。验证试验表明,饱和水溶液法制备AA-2G-β-CD包合物工艺稳定。通过傅里叶红外色谱法对制备的AA-2G-β-CD包合物进行了鉴定,证明了AA-2G-β-CD包合物的形成。通过抗氧化性实验发现,包合物清除氧自由基能力高于AA-2G与β-CD混合物。综上,采用饱和水溶液法制备AA-2G-β-CD包合物,经验证AA-2G-β-CD包合物形成,通过正交实验优化制备工艺后,其包合率达到49.55%,同时包合物的抗氧化性能力高于AA-2G与β-CD混合物。In order to improve the stability and bioavailability of 2-O-α-D-glucopyranosyl-L-ascorbic acid(AA-2G),the inclusion compound of AA-2G in beta-cyclodextrin(β-CD)was prepared.The preparation process of AA-2G-β-CD inclusion compound was studied by saturated aqueous solution method.The effects of temperature,time,stirring speed and the molar ratio ofβ-CD to AA-2G on the preparation of inclusion compound were investigated by single factor experiments.The optimized conditions for the preparation of AA-2G-β-CD inclusion compound were determined by orthogonal experiments listed as follows:the molar ratio of AA-2G toβ-CD as 1:3,temperature as 60°C,the stirring speed as 200 r/min,time as 5 h,and the resulting inclusion rate was 49.55%.The order of factors affecting inclusion ratio was time>temperature>speed>molar ratio.The validation test showed that the preparation of AA-2G-β-CD inclusion compound by saturated aqueous solution method was stable.The AA-2G-β-CD inclusion compound was identified by FT-IR,proving the formation of AA-2G-β-CD inclusion compound.It was found that the free radical scavenging ability of the inclusion compound was higher than that of the AA-2G andβ-CD mixture.In summary,AA-2G inclusion compound was prepared by saturated aqueous solution method.The formation of AA-2G-β-CD inclusion compound was confirmed.The inclusion rate of AA-2G-β-CD inclusion compound reached 49.55%after optimizing the preparation process by orthogonal experiments.At the same time,the antioxidant ability of the inclusion compound was higher than that of the AA-2G andβ-CD mixture.

关 键 词：抗坏血酸葡萄糖苷 Β-环糊精 包合物 红外色谱 自由基清除率 

分 类 号：TQ4[化学工程]