双水相体系萃取纯化紫甘蓝色素  

Separation of anthocyanidins from red cabbage by aqueous twophase system

作　　者：李治城 李靖靖 潘晓静 展亚莉 常秀莲[1] LI Zhi-cheng;LI Jing-jing;PAN Xiao-jing;ZHAN Ya-li;CHANG Xiu-lian(School of Life Sciences,Yantai University,Yantai 264005;Qindao Pengyuan Kanghua Natural Products Company,Co.Ltd.,Laixi 266612)

机构地区：[1]烟台大学生命科学学院,烟台264005 [2]青岛鹏远康华天然产物有限公司,莱西266612

出　　处：《中国食品添加剂》2019年第9期77-81,共5页China Food Additives

基　　金：横向课题（SK18H08,SK18H09,SK19H21）;烟台大学2019年开放实验室（从紫甘蓝废渣中分离果胶）

摘　　要：采用聚乙二醇(PEG)/硫酸铵双水相体系萃取纯化紫甘蓝中的花青素。通过单因素实验优化了硫酸铵、PEG、粗提液的质量分数和体系的pH,通过正交实验确定各因素对花青素选择性系数的影响程度,粗提液的质量分数>pH>硫酸铵的质量分数>PEG的质量分数。实验最佳提取条件为:粗提液的质量分数为12%,硫酸铵质量分数为20%,pH为3.5,PEG的质量分数为15%,花青素的选择性系数为750.26。The anthocyanins in red cabbage were extracted by PEG/ammonium sulfate aqueous two-phase system.The mass fraction of ammonium sulfate,PEG,crude extracts and the pH value of the system were optimized by single factor experiment.The effect of various factors on the selectivity coefficient of anthocyanins were determined by orthogonal experiment in an order of crude extract mass fraction>pH>mass fraction of ammonium sulfate>mass fraction of PEG.The optimum extraction conditions were as follows:the crude extracts 12%,the ammonium sulfate 20%,pH 3.5,PEG 15%.Under the above conditions,the anthocyanin selectivity coefficient was 750.26.

关 键 词：紫甘蓝 天然色素 双水相萃取 选择性系数 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS264.4[轻工技术与工程—食品科学与工程]