西藏热泉1株产纤维素酶真菌的鉴定及产酶条件优化  

Identification of a Cellulase Producing Fungus from Tibet Hot Spring and Optimization of Its Enzyme Production Conditions

作　　者：卢雨欣 赵航轲 唐小飞[1] 刘开辉[1,2] 邓百万 丁小维[1,2] LU Yuxin;ZHAO Hangke;TANG Xiaofei;LIU Kaihui;DENG Baiwan;DING Xiaowei(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China;School of Food and Biological Engineering,Shaanxi University of Science&Technology,Xi’an 710021,China;Shaanxi Provincial Engineering Research Center of Edible and Medicinal Fungi,Hanzhong 723000,China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]陕西科技大学食品与生物工程学院,陕西西安710021 [3]陕西省食药用菌工程技术研究中心,陕西汉中723000

出　　处：《河南农业科学》2019年第10期77-83,共7页Journal of Henan Agricultural Sciences

基　　金：陕西省科技厅重点研发计划项目(2018NY-156)

摘　　要：为深化嗜热纤维素酶资源的开发利用,从西藏尼木热泉分离得到1株产纤维素酶嗜热真菌THN8,根据该菌株在刚果红培养基上产生的透明圈大小,初步判断其产纤维素酶能力。通过形态学和ITS序列分析,鉴定其为Melanocarpus albomyces。在单因素试验基础上采用正交试验确定菌株THN8产纤维素酶的最佳培养基配方及发酵条件,最终获得该菌株产纤维素酶的最佳培养基配方为乳糖92.0 g/L、(NH 4)2SO 4 6.0 g/L、K 2HPO 4 3.0 g/L、MgSO 4·7H 2O 0.55 g/L,最佳发酵条件为发酵温度58℃、起始pH值5.5、发酵时间4 d,在此条件下菌株THN8的纤维素酶活力达到28.1 U/mL。试验分离出的产纤维素酶嗜热真菌在降解利用纤维素类农业废弃物方面具有重要作用。In order to deepen the development and utilization of thermophilic cellulase resources,a cellulase producing fungus THN8 from Nimu hot spring in Tibet was isolated and identified,and the cellulase producing activity of this strain was screened by Congo red culture medium.According to the morphology and ITS sequence analysis,the strain THN8 was identified as Melanocarpus albomyces.On the basis of single factor experiment,orthogonal experiment was used to optimize the cellulase producing conditions of the strain THN8.Finally,the optimal medium formula for the cellulase producing strain THN8 was as follows:lactose 92.0 g/L,(NH 4)2SO 4 6.0 g/L,K 2HPO 4 3.0 g/L,MgSO 4·7H 2O 0.55 g/L.The optimal fermentation conditions were as follows:the fermentation temperature was 58℃,the initial pH value was 5.5,and the fermentation time was 4 days.Under these conditions,the cellulase activity of the strain THN8 reached 28.1 U/mL.The thermophilic fungus isolated in this experiment plays an important role in the degradation and utilization of cellulose agricultural waste.

关 键 词：西藏热泉 嗜热真菌 鉴定 纤维素酶 产酶条件 

分 类 号：S154[农业科学—土壤学]