发酵法制备沙棘籽粕总黄酮的初步纯化及抗衰老活性评价  

Preliminary purification and anti-aging activity evaluation of total flavonoids prepared from sea-buckthorn seed meal by fermentation

作　　者：由士权 王昌涛[1,2] 张佳婵[1] 李萌[1] 赵丹 安全 YOU Shi-quan;WANG Chang-tao;ZHANG Jia-chan;LI Meng;ZHAO Dan;AN Quan(Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,China;Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University,Beijing 100048,China;Yunnan Baiyao Group Health Products Co.Ltd.,Kunming,Yunnan 650500,China)

机构地区：[1]北京工商大学植物资源研究开发北京市重点实验室,北京100048 [2]北京工商大学北京食品营养与人类健康高精尖创新中心,北京100048 [3]云南白药集团健康产品有限公司,云南昆明650500

出　　处：《日用化学工业》2019年第10期661-667,共7页China Surfactant Detergent & Cosmetics

基　　金：北京工商大学两科基金培育项目（LKJJ2016-09）;北京工商大学学位与研究生教育研究项目（2017XWYJS014）

摘　　要：研究大孔吸附树脂纯化沙棘籽粕发酵提取液的工艺条件以及抗衰老活性。对3种大孔吸附树脂分离纯化的效果进行了比较,考察了X-5大孔树脂分离的较佳工艺条件,并通过MTT法评价了提取物对细胞生长的影响,采用DPPH法、提取物对细胞内活性氧和MMP-1活性的抑制、促进胶原蛋白合成来评价提取物的抗衰老活性。结果表明,较佳工艺条件为:吸附时间2h,吸附温度15℃,洗脱剂体积分数60%,洗脱时间2.5h,洗脱温度45℃。在此条件下吸附量可达2.33 mg/g,解析率可达94.75%;MTT实验表明对成纤维细胞基本无毒副作用;对DPPH自由基清除率达到50%时的质量浓度(IC50)为1.06 mg/L,活性氧水平显著下降,MMP-1活性降低,胶原蛋白含量有所提高。沙棘籽粕发酵液有较好的抗衰老活性。The fermentation extract of sea-buckthorn seed meal was purified by macroporous adsorption resin.The technological conditions in the purification process were studied,as well as the anti-aging activity of the extract.The effects of separation and purification of three macroporous adsorption resins were compared.The optimum separation conditions with X-5 macroporous resin were investigated.The effect of the extract on cell growth was evaluated by MTT method.The anti-aging activity of the extract was evaluated by DPPH method,the inhibition of the activities of intracellular reactive oxygen species and MMP-1,and the promotion of collagen synthesis.The results show that the optimum process conditions:adsorption time of 2 h,adsorption temperature of 15℃,eluent volume fraction of 60%,elution time of 2.5 h,and elution temperature of 45℃.Under such conditions,the adsorption amount could reach 2.33 mg/g,and the resolution rate could reach 94.75%.The MTT experiment showed that it had no toxic side effects on fibroblasts;the mass concentration when the DPPH radical scavenging rate reaches 50%(IC50)was 1.06 mg/L;the level of reactive oxygen species decreased significantly;the MMP-1 activity was decreased;the collagen content was increased.The fermentation broth of sea-buckthorn seed meal showed good anti-aging activity.

关 键 词：化妆品添加剂 沙棘籽粕 黄酮 发酵 抗衰老 

分 类 号：TQ658[化学工程—精细化工]