不同产地党参多糖含量及抗氧化活性比较  

Comparison of Polysaccharide Content and Antioxidant Activity of Codonopsis pilosula in Different Producing Areas

作　　者：叶英[1] 李珊 张景宜 赵永珍 哈生云 刘圆[3] YE Ying;LI Shan;ZHANG Jing-yi(College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海圣航农牧科技开发有限公司,青海尖扎811200 [3]西南民族大学民族医药研究院,四川成都610041

出　　处：《安徽农业科学》2019年第21期184-188,共5页Journal of Anhui Agricultural Sciences

基　　金：青海省科技合作专项(2016-HZ-821)

摘　　要：[目的]比较不同产地党参多糖含量及抗氧化活性。[方法]采用单因素和正交试验设计优化党参多糖提取工艺条件,对不同产地党参多糖的含量进行比较,并采用ABTS、DPPH自由基清除率试验及铁离子还原法对不同产地党参多糖抗氧化活性进行比较。[结果]超声辅助法提取党参多糖的最佳工艺条件为超声温度60℃、超声时间40 min、料液比1∶30、提取次数3次;此条件下,吉林长白山的党参多糖得率最高,达29.56%,其次是青海尖扎党参,多糖含量达22.38%。不同产地党参多糖对ABTS自由基清除能力从大到小依次为甘肃定西、青海尖扎、青海大通、四川雅安、山西太行、吉林长白山,对DPPH自由基清除能力从大到小依次为甘肃定西、青海大通、青海尖扎、山西太行、吉林长白山、四川雅安,铁离子还原能力从大到小依次为青海大通、甘肃定西、吉林长白山、山西太行、四川雅安、青海尖扎。[结论]该研究为党参多糖的质量品质评价及抗氧化功能性食品的研究开发提供理论依据。[Objective]The research aimed to compare the polysaccharide content and antioxidant activity of Codonopsis pilosula in different producing areas.[Method]The extraction conditions of Codonopsis pilosula polysaccharides were optimized by single factor and orthogonal design.The content of Codonopsis pilosula polysaccharides from different producing areas was compared,and the antioxidant activities of Codonopsis pilosula polysaccharides were compared by ABTS free radical,DPPH free radical scavenging rate test and iron ion reduction method.[Result]The optimum conditions for ultrasonic extraction of Codonopsis pilosula polysaccharides were as follows:ultrasonic temperature 60℃,ultrasonic time 40 min,solid-liquid ratio 1∶30,extraction times 3 times.Under the optimum extraction conditions,the yield of polysaccharide from Codonopsis pilosula was 29.56%in Changbai Mountain of Jilin Province,followed by Codonopsis pilosula in Jianzha of Qinghai Province,and the content of polysaccharide was 22.38%.The scavenging capacity of Codonopsis pilosula polysaccharides from different producing areas to ABTS free radicals from large to small was Gansu Dingxi,Qinghai Jianzha,Qinghai Datong,Sichuan Ya’an,Shanxi Taihang,Jilin Changbai Mountain.The scavenging capacity of DPPH free radicals from large to small was Gansu Dingxi,Qinghai Datong,Qinghai Jianzha,Shanxi Taihang,Jilin Changbai Mountain,Sichuan Ya’an,and iron reduction capacity from large to small was Qinghai Datong,Gansu Dingxi,Jilin Changbai Mountain,Shanxi Taihang,Sichuan Ya’an,Qinghai Jianzha.[Conclusion]The research provides theoretical basis for the quality and quality evaluation of Codonopsis pilosula polysaccharides and the research and development of antioxidant functional foods.

关 键 词：党参多糖 不同产地 含量 抗氧化活性 

分 类 号：R[医药卫生]