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作 者:廖先清[1] 刘芳[1] 周荣华[1] 饶犇 陈伟[1] 王开梅[1] LIAO Xian-qing;LIU Fang;ZHOU Rong-hua;RAO Ben;CHEN Wei;WANG Kai-mei(Hubei Biopesticide Engineering Research Center/Biopesticide Subcenter of Hubei Science and Technology Innovation Center,Wuhan 430064,China)
机构地区:[1]湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药研究分中心
出 处:《湖北农业科学》2019年第20期97-100,共4页Hubei Agricultural Sciences
基 金:国家重点研发计划课题(2017YFD0201205);湖北省农业科技创新中心资助项目(2016-620-000-001-038)
摘 要:为获得一株侧孢芽孢杆菌(Bacillus laterosporus)(NBF-129)发酵工艺,以发酵液活芽孢数为指标,在单因素试验基础上,采用正交试验对其培养基及培养条件进行优化。培养基优化结果为玉米淀粉2.0%、蚕蛹粉2.5%、酵母浸出粉1.0%、玉米浆1.0%。最佳培养条件为初始pH 7.5,培养温度30℃,转速220 r/min。优化后的侧孢芽孢杆菌摇瓶发酵液芽孢数达到4.12×10^9 CFU/mL,较优化前的2.30×10^9 CFU/mL提高79.1%。利用优化后的培养基和培养条件进行了30 L罐发酵小试,发酵周期为35 h,发酵液芽孢数为3.96×10^9 CFU/mL,发酵液离心浓缩后菌浆冷冻干燥,得到原粉芽孢数为6.70×10^10 CFU/g。^In order to obtain the fermentation process of a strain of Bacillus laterosporus NBF-129,the culture medium and conditions were optimized by single factor experiment and orthogonal test,using the number of viable spores in the fermentation broth as an index.The optimal fermentation medium were(%):corn starch 2.0,silkworm pupa meal 2.5,yeast extract 1.0,corn steep liqour 1.0.The optimal fermentation conditions were:initial pH 7.5,culturing temperature 30℃,rotation speed 220 r/min.After optimization,the number of viable spores in the fermentation broth reached 4.12×10^9 CFU/mL,which was 79.1%higher than 2.30×10^9 CFU/mL before optimization.Under the optimized medium and optimal culturing conditions,a 30-liter tank fermentation test was conducted.The incubation time was 35 hours,The number of viable spores in the fermentation broth reached 3.96×10^9 CFU/mL.After being concentrated and freeze-dried,the number of raw powder spores was 6.70×10^10 CFU/g.
关 键 词:侧孢芽孢杆菌(Bacillus laterosporus) 发酵 培养基优化 正交试验
分 类 号:Q93[生物学—微生物学] TQ920.6[轻工技术与工程—发酵工程]
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