磁性壳聚糖微球的制备及表征  

Preparation and characterization of magnetic chitosan microspheres

作　　者：李黎[1] 马力[2] 李鹤[2] Li Li;Ma Li;Li He(Chengdu Agricultural College,Chengdu 611130,Sichuan Province,China;Xihua University,Chengdu 610039,Sichuan Province,China)

机构地区：[1]成都农业科技职业学院,四川省成都市611130 [2]西华大学,四川省成都市610039

出　　处：《中国组织工程研究》2020年第4期577-582,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:磁性高分子微球作为酶、细胞、药物等的载体被广泛地应用到了生物工程、细胞学和生物医学等领域。目的:制备出粒径小、分散性好、磁响应强、安全无毒的磁性高分子微球。方法:以Fe3O4磁性粒子为内核、液体石蜡为分散介质、Span-80为乳化剂、戊二醛为交联剂,采用反相悬浮交联法制备磁性壳聚糖微球,探讨了交联时间(0,20,40,60,80,100,120,150,180min)、反应温度(20→50℃、30→60℃、40→70℃、50→80℃)、壳聚糖质量浓度(0.01,0.02,0.03,0.04,0.05g/mL)、Fe3O4/壳聚糖质量比(1∶1,1∶2,1∶3,1∶4)、戊二醛用量(8-10 mL)、液体石蜡用量(40,60,80,100 mL)、搅拌速度(0-2 000 r/min)等因素对磁性壳聚糖微球性能的影响,并对其形态、粒径、分散性、磁响应性进行了表征。结果与结论:(1)实验发现制备磁性壳聚糖微球的最佳条件为:从加入交联剂戊二醛开始计时,先于40℃反应1 h再升温至70℃继续反应120 min,壳聚糖浓度为0.02 g/mL,Fe3O4/壳聚糖质量比为1∶2,液体石蜡用量为80 mL,搅拌速度为1 200 r/min,戊二醛用量为8-10 mL;(2)磁性壳聚糖微球在外加磁场下具有强磁性能,在自然状态下具有良好的悬浮稳定性;(3)壳聚糖与Fe3O4纳米粒子形成的复合微粒呈球状,纳米粒子被包裹在微球内,为核壳结构,微球表面较平滑,单分散性好;(4)制备的磁性壳聚糖微球粒径介于1-15μm之间,该粒径利于微球在反应体系中分散和磁分离。BACKGROUND: As carriers of enzymes, cells and drugs, magnetic polymer microspheres have been widely used in the fields of bioengineering, cytology, and biomedicine. OBJECTIVE: To prepare the magnetic polymer microspheres characterized by small particle size, good dispersion, strong magnetic response, safety, and non-toxicity. METHODS: Magnetic chitosan microspheres were prepared by reverse phase suspension process using Fe3O4 as core, paraffin as dispersed medium, Span-80 as emulsifier, and glutaraldehyde as cross-linking agent. The effects of factors including crosslinking time(0, 20, 40, 60, 80, 100, 120, 150 and 180 minutes), reaction temperature(20→50℃, 30→60℃, 40→70℃, 50→80℃), the concentration of chitosan(0.01, 0.02, 0.03, 0.04, 0.05 g/mL), Fe3O4/chitosan mass ratio(1∶1, 1∶2, 1∶3, 1∶4), the amount of glutaraldehyde(8-10 mL), theamount of liquid paraffin(40, 60, 80, 100 mL), and stirring speed(0-2 000 r/min) on the properties of magnetic chitosan microspheres. The morphology, particle size, dispersion, and magnetic responsiveness of magnetic chitosan microspheres were characterized. RESULTS AND CONCLUSION: The optimum conditions for preparing magnetic chitosan microspheres were as follows: starting with glutaraldehyde as crosslinking agent, the reaction was performed at 40℃ for 1 hour and then at 70℃ for 120 minutes. The concentration of chitosan was 0.02 g/mL, the mass ratio of Fe3O4/chitosan was 1∶2, the dosage of liquid paraffin was 80 mL, the stirring speed was 1 200 r/min, and the dosage of glutaraldehyde was 8-10 mL. Magnetic chitosan microspheres had strong magnetic properties under the applied magnetic field and had good suspension stability in the natural state. The Fe3O4/chitosan composites were spherical, and the nanoparticles were encapsulated in the microspheres, which were core-shell structure. The surface of the microspheres was smooth and monodisperse. The magnetic chitosan microspheres prepared had a diameter of 1-15 μm, which is beneficial to the dispersion an

关 键 词：磁性微球 高分子微球 生物材料 壳聚糖 固定化载体 磁响应 反相悬浮交联法 四氧化三铁 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]