Protective effects and potential underlying mechanisms of sodium copper chlorophyllin against ethanol-induced gastric ulcer in mice  

作　　者：Huawei Lv Yan Lin Peigang Liu Weiqing Liang Kemin Wei Jinbao Pu Hongjian Zhang 

机构地区：[1]College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China [2]Department of Pharmacy,Tongde Hospital of Zhejiang Province,Hangzhou 310012,China [3]Center for Medicinal Resources Research,Key Laboratory of Research and Development of Chinese Medicine of Zhejiang Province,Zhejiang Academy of Traditional Chinese Medicine,Hangzhou 310007,China

出　　处：《Acta Biochimica et Biophysica Sinica》2019年第9期925-933,共9页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants of the Program of Zhejiang Provincial Department of Science and Technology(No.2016C31001);the Project of Administration of Traditional Chinese Medicine of Zhejiang Province(No.2016ZQ001);the National Natural Science Foundation of China(No.81603255).

摘　　要：In study,we aimed to determine the mechanisms underlying the gastroprotective effects of sodium copper chlorophyllin(SCC)against ethanol-induced gastric ulcer injury in mice.First,the gastroprotective effects of SCC against gastric ulcer induced by ethanol were assessed.Then,biochemical,histopathological,immunohistochemistry assays,and western blot analysis were conducted to determine the possible mechanisms of action underlying the effects of SCC.Compared to the effects of omeprazole(OME)in a confirmed mouse model of ethanol-induced gastric ulcer injury,treatment with various doses of SCC resulted in up-regulation of Bcl-2 and down-regulation of the pro-apoptotic protein Bax.Significant decreases in the levels of the malondialdehyde(MDA),myeloperoxidase(MPO),and NO in the gastric tissues were observed.Furthermore,inflammatory cytokine analysis revealed that SCC treatment inhibited the expressions of TNF-αand IL-6,greatly reduced the phosphorylation level of IκB,and repressed the nuclear translocation of NF-κB p65,which demonstrated that SCC inhibited the activation of the NF-κB pathway.The present findings suggest that the protective effects of SCC may be beneficial as a potential preventive and therapeutic agent for gastric ulcer through the NF-κB pathway.Taken together,SCC administration significantly decreased the levels of MPO,NO,and MDA in gastric tissue and exerted a powerful anti-inflammatory activity as demonstrated by reduction in the secretions of proinflammatory mediators such as IL-6 and TNF-α in the serum of mice exposed to ethanol.

关 键 词：sodium copper CHLOROPHYLLIN gastro protection OXIDATIVE stress PROINFLAMMATORY apoptosis 

分 类 号：R73[医药卫生—肿瘤]