猴头菇多糖和蛋白质闪式联合提取工艺优化及结构鉴定  

Optimization of flash extraction process and structure identification of polysaccharide and protein from Hericium erinaceus

作　　者：张雄 肖志勇 黄群[1] 黄翔 杨燃 陈雷[1] 王艺伟[1] ZHANG Xiong;XIAO Zhi-yong;HUANG Qun;HUANG Xiang;YANG Ran;CHEN Lei;WANG Yi-wei(College of Food Science Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Tuotian Biological Technology Company,Fuzhou,Fujian 350001,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建拓天生物科技有限公司,福建福州350001

出　　处：《食品与机械》2019年第10期117-121,183,共6页Food and Machinery

基　　金：福建省科技计划项目（编号：2019N0007）;福州市科技计划项目（编号：2018-G-33)

摘　　要：采用闪式提取法同步提取猴头菇中多糖和蛋白质,在单因素试验基础上利用响应面优化提取工艺条件,并分别采用红外光谱和SDS-PAGE电泳分析多糖的结构及蛋白质分子的亚基组成。结果表明,闪式法同步提取猴头菇中多糖和蛋白质的最佳工艺参数为:提取时间70 s、液料比17 1(mL/g)、粉体粒度90目、提取电压100 V,猴头菇多糖提取得率达(9.24±0.15)%,蛋白质得率为1.33%。经红外光谱分析猴头菇多糖在3 400,2 930,1 400~1 200 cm^-1附近有吸收峰;SDS-PAGE电泳分析猴头菇蛋白质主要分布在44.3,66.4 kDa附近。采用闪式提取法可同步提取猴头菇中多糖和蛋白质,且能有效保护天然产物的活性成分。The polysaccharides and proteins from Hericium erinaceus were flashly extracted.On the basis of single factor experiments,the response surface methodology was applied to optimize the extraction conditions,and the structure of the polysaccharide and the subunit composition of the protein molecule were analyzed by infrared spectroscopy and SDS-PAGE respectively.The optimal condition for extraction of Hericium erinaceus polysaccharides(HEP)and proteins were:extracting time 80 s,liquid-solid ratio 17 1(mL/g),grinding granularity 80 meshes,extracting voltage 100 V,HEP yield rate(9.24±0.15)%and protein yield rate was 1.34%.The fourier transform infrared spectrum showed that HEP had absorption peaks near 3 400,2 930 and 1 400~1 200 cm^-1;the protein mass was mainly distributed around 44.3 and 66.4 kDa by SDS-PAGE electropho-retic.The results will provide a feasible way and theoretical reference for the study on polysaccharides and proteins of Hericium erinaceus.

关 键 词：猴头菇 闪式提取法 多糖 蛋白质 同步提取 结构鉴定 

分 类 号：S64[农业科学—蔬菜学]