黑莓天然维生素E纯化技术研究  

Study on purification technology of natural vitamin E in blackberry

作　　者：李维林 崔恩惠[2] 赵慧芳[2] 方亮[2] 吴文龙[2] LI Wei-Lin;CUI En-Hui;ZHAO Hui-Fang;FANG Liang;WU Wen-Long(College of Forestry,Nanjing Forestry University,Co-Innovation Centerfor the Sustainable Forestryin Southern China,Nanjing 210037,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China)

机构地区：[1]南京林业大学林学院,南方现代林业协同创新中心,南京210037 [2]江苏省中国科学院植物研究所,南京210014

出　　处：《食品安全质量检测学报》2019年第19期6611-6614,共4页Journal of Food Safety and Quality

基　　金：江苏省农业科技自主创新项目(CX(18)2018);江苏现代农业(特粮特经)产业技术体系项目(SXGC[2018]309)~~

摘　　要：目的建立一种硅胶柱层析纯化黑莓种籽油中天然VE的方法。方法采用单因素实验的方法,以维生素E的含量为考察指标,对洗脱剂种类、洗脱剂配比、硅胶用量、洗脱剂用量进行优化。结果黑莓天然VE的最优纯化工艺参数为:硅胶与维生素E粗提物的质量比3:1;乙醇?正己烷(8:1,V/V)为洗脱剂,洗脱剂用量为粗提物的15倍。在此条件下,纯化物中维生素E的含量可以达到(51.5±0.530)%。结论采用硅胶柱层析方法可以有效纯化黑莓天然维生素E。Objective To establish a method for purifying VE from blackberry seed oil by silica gel column chromatography. Methods Single factor experiments were carried out to optimize the purification process of natural VE from blackberry seed oil. The eluent type, ethanol/hexane ratio, silica gel weight and eluent volume were selected by taking VE content as the index. Results The optimal purification process parameter of berry natural VE was: Silica gel and the quality of the crude extracted from vitamin E than 3?1;Ethanol : n-hexane(8?1, V/V) as eluent, dosage of eluent for the crude extracted of 15 times. Under these conditions, the content of vitamin E in the purified compound could reach(51.5±0.530)%. Conclusion Silica gel column chromatography is a feasible method for the purification of blackberry natural Vitamin E.

关 键 词：黑莓 维生素E 纯化 

分 类 号：R28[医药卫生—中药学]