微波消解-电感耦合等离子体质谱法测定食用菌基质中4种重金属  

Determination of 4 kinds of heavy metals in edible fungi by microwave digestion and inductively coupled plasma-mass spectrometry

作　　者：刘烨潼 张强 殷萍 刘璐 苏芳 孙瑞 陈秋生 LIU Ye-Tong;ZHANG Qiang;YIN Ping;LIU Lu;SU Fang;SUN Rui;CHEN Qiu-Sheng(Tianjin Institute of Agricultural Quality Standard and Testing Technology,Tianjin 300381,China)

机构地区：[1]天津市农业质量标准与检测技术研究所

出　　处：《食品安全质量检测学报》2019年第20期6950-6956,共7页Journal of Food Safety and Quality

基　　金：天津市农业科技成果转化与推广项目(201701080)~~

摘　　要：目的建立一种微波消解-电感耦合等离子质谱法(inductivelycoupledplasma-massspectrometry,ICP-MS)测定食用菌基质中有害重金属砷(As)、汞(Hg)、铅(Pb)和镉(Cd)含量的方法。方法采用硝酸和过氧化氢对食用菌基质样品进行微波消解,消解液经稀释后用电感耦合等离子体质谱仪测定,在线内标法定量,采用国家标准物质土壤(GBW07427)和杨树叶(GBW07604)以及重复性试验,幵经过3个水平的加标回收试验进行验证。结果测定的不同品种食用菌基质中的4种重金属元素的加标回收率在90.7%~105.3%乊间,相对标准偏差在0.88%~4.03%乊间。结论该方法稳定性好、灵敏度高、定量准确,提高了检测效率,适用于食用菌基质中As、Hg、Pb和Cd, 4种重金属快速、准确的定量分析。Objective To establish a method for the determination of heavy metals, including plumbum(Pb), cadmium(Cd), mercury(Hg) and arsenic(As) in edible fungi by microwave digestion and inductively coupled plasma-mass spectrometry(ICP-MS). Methods Nitric acid and hydrogen peroxide were used for microwave digestion for edible fungi samples. The digestion solution was diluted and determined by ICP-MS, and quantified by on-line internal standard method. It was verified by national standard material soil(GBW07427) and poplar leaves(GBW07604) and repeatability test and 3 levels of spike recovery test. Results The recoveries of 4 heavy metals in different edible fungus matrix were between 90.7% and 105.3%, and the relative standard deviations(RSDs) were between 0.88% and 4.03%. Conclusion This method has good stability, high sensitivity, accurate quantification and improves detection efficiency. It is suitable for the rapid and accurate quantitative analysis of As, Hg, Pb and CD in edible fungi matrix.

关 键 词：食用菌 微波消解 电感耦合等离子质谱 重金属 检测 

分 类 号：S64[农业科学—蔬菜学]