棒状链霉菌产克拉维酸发酵培养基优化  

Optimization in Fermentation Medium for Clavulanic Acid Production by Streptomyces clavuligerus

作　　者：冯涛[1,2] 苗瑞春 李雄 王雁 杨波 曹斌 刘新山 王永红 储炬[1] FENG Tao;MIAO Ruichun;LI Xiong;WANG Yan;YANG Bo;CAO Bin;LIU Xinshan;WANG Yonghong;CHU Ju(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;Sinopharm Weiqida Pharmaceutical Co.,Ltd.,Datong 037300,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]国药集团威奇达药业有限公司,山西大同037300

出　　处：《化学与生物工程》2019年第11期12-15,25,共5页Chemistry & Bioengineering

基　　金：国家科技重大专项重大新药创制项目(2011ZX09203)

摘　　要：以克拉维酸生产菌株Streptomyces clavuligerus K6为研究对象,考察了不同氮源对克拉维酸产量的影响,并通过二水平析因实验、快速爬坡实验和响应面法对发酵培养基碳源进行优化。确定最佳氮源为大豆蛋白,最佳发酵培养基碳、氮源配方为:甘油18.19 g·L^-1、糊精11.22 g·L^-1、三油酸甘油酯30.80 g·L^-1、大豆蛋白粉40 g·L^-1。在此发酵培养基配方下,克拉维酸产量可达5220 mg·L^-1,较出发配方提高58.1%。Using clavulanic acid-producing strain Streptomyces clavuligerus K6 as a research subject,we investigated the effects of different nitrogen sources on clavulanic acid production,and optimized the carbon source of fermentation medium by two-level fractional factorial experiments,steepest ascent experiments,and response surface methodologies.The results show that the optimal nitrogen source is soy protein;meanwhile,the optimal carbon source and nitrogen source of fermentation medium are determined as follows:the glycerol of 18.19 g·L^-1,the dextrin of 11.22 g·L^-1,the triolein of 30.80 g·L^-1,and the soy protein of 40 g·L^-1.In above fermentation medium,the clavulanic acid production can reach 5 220 mg·L^-1,which is increased by 58.1% compared with that of the original medium.

关 键 词：克拉维酸 发酵 棒状链霉菌 响应面法 

分 类 号：TQ927[轻工技术与工程—发酵工程]