碱溶酸沉法提取梧桐子蛋白质的工艺  

Extraction of Chinese Parasol Seed Protein by Alkali Extraction and Acid Precipitation

作　　者：王萍[1] 位治国[2] 郭金英[1] 崔国庭[1] WANG Ping;WEI Zhiguo;GUO Jinying;CUI Guoting(College of Food&Bioengineering,Henan University of Science and Technology,Luoyang 471023;College of Animal Science&Technology,Henan University of Science and Technology,Luoyang 471023)

机构地区：[1]河南科技大学食品与生物工程学院,洛阳471023 [2]河南科技大学动物科技学院,洛阳471023

出　　处：《食品工业》2019年第10期110-113,共4页The Food Industry

基　　金：河南省教育厅自然科学研究项目（15A180035）;河南科技大学校基金项目（2014QN066）;国家自然科学基金青年科学基金项目（31100886）;博士启动费（13480033）

摘　　要：以梧桐子为原料,以蛋白质提取率为指标,通过单因素和正交试验,确定碱溶酸沉法提取梧桐子蛋白质的最佳工艺参数。结果表明,影响梧桐子蛋白质提取率的因素大小依次为:浸提时间、浸提温度、料液比和碱提液p H。碱溶酸沉法提取梧桐子蛋白质的最佳工艺参数为:碱提液pH 9、料液比1︰15(g/mL)、浸提时间120 min、浸提温度40℃。此条件下,梧桐子蛋白质的提取率为31.36%;梧桐子中蛋白质提取液的最佳酸沉pH为3.8。The extraction process of protein from Chinese parasol seed was investigated by using alkaline dissolution and acid precipitation method.The optimum parameters were determined through the single factor and orthogonal experiments,with the extraction rate of protein served as the reference.The results showed that the main factors affecting the extraction rate of the protein Chinese parasol seed were the extraction time>leaching temperature>the ratio of material to liquid>alkali extraction pH.The optimum extraction parameters were as follows:pH 9,the ratio of material to liquid 1︰15(g/m L),the extraction time 120 min,and the extraction temperature 40℃.Under this technological condition,the extraction rate of protein from Chinese parasol seed was 31.36%,and the best acid precipitation pH was 3.8.

关 键 词：梧桐子 蛋白质 提取率 碱溶酸沉 

分 类 号：TS2[轻工技术与工程—食品科学与工程]