液相色谱–同位素稀释质谱法测定婴幼儿配方奶粉中黄曲霉毒素M1  

Determination of aflatoxin M1 in infant formula milk powder by liquid chromatography isotope dilution mass spectrometry

作　　者：魏敏 李兴佳 周剑[2] 柴艳兵 张耀广 李飞 WEI Min;LI Xingjia;ZHOU Jian;CHAI Yanbing;ZHANG Yaoguang;LI Fei(Junlebao Dairy Group,Shijiazhuang Key Laboratory of Dairy Quality and Safety Control,Ministry of Agriculture and Villages,Shijiazhuang 050021,China;Key Laboratory of AgrifoodSafety and Quality,Ministry of Agriculture and Rural Affairs,Institute of Quality Standard and Testing Technology for Agro-Products of CAAS,Beijing 100081,China)

机构地区：[1]君乐宝乳业集团农业农村部乳品质量安全控制重点实验室,石家庄050021 [2]中国农业科学院农业质量标准与检测技术研究所农业农村部农产品质量安全重点实验室,北京100081

出　　处：《化学分析计量》2019年第6期1-5,共5页Chemical Analysis And Meterage

基　　金：国家重点研发计划项目(SQ2018YFC1604302)

摘　　要：建立液相色谱-同位素稀释质谱法测定婴幼儿配方奶粉中黄曲霉毒素M1基体标准物质研制的定值方法。采用单点逼近法,以13C17-AFTM1为内标,乙腈为溶剂,经免疫亲和柱净化,采用单点逼近法测定婴幼儿配方奶粉中黄曲霉毒素M1的含量。黄曲霉毒素M1的质量浓度在0.05~5.0 ng/mL范围内与色谱峰面积线性关系良好,线性相关系数为0.9993,平均回收率为102.0%。该方法稳定性好,操作简单,可以作为婴幼儿配方奶粉中黄曲霉毒素M1基体标准物质研制的定值方法。A method for the determination of aflatoxin M1 in infant formula milk powder by liquid chromatography-isotope dilution mass spectrometry was established.The content of aflatoxin M1 in infant formula milk powder was determined by single point approximation method with 13C17-aftm1 as internal standard,acetonitrile was used purified by as solvent and immunoaffinity column.The mass concentration of aflatoxin M1 in the range of 0.05-5.0 ng/mL has a good linear relationship with the peak area of chromatographic spectrometry.The linear correlation coefficient was 0.9993,and the average recovery was 102.0%.The method has good stability and simple operation,and can be used as a fixed value method for the development of aflatoxin M1 matrix reference material in infant formula milk powder.

关 键 词：婴幼儿配方奶粉 黄曲霉毒素M1 基体标准物质 定值方法 

分 类 号：O652.3[理学—分析化学]