QuEChERS-超高效液相色谱串联质谱法测定玉米中的17种真菌毒素  

Determination of 17 Mycotoxins in Maize by QuEChERS-ultra High Performance Liquid Chromatography Tandem Mass Spectrometry

作　　者：姜涛[1] 赵琳 兰静 钱镭[1] 王冰 贾雯静 张瑞英 JIANG Tao;ZHAO Lin;LAN Jing;QIAN Lei;WANG Bing;JIA Wen-jing;ZHANG Rui-ying(Department of Food and Environmental,East University of Heilongjiang,Harbin 150066,China;Institute of Agricultural Product Quality and Safety,Heilongjiang Academy of Agricultural Sciences,Laboratory of Agricultural Product Quality and Safety Risk Assessment,Ministry of Agriculture and Rural Agriculture,Harbin 150086,China)

机构地区：[1]黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150066 [2]黑龙江省农业科学院农产品质量安全研究所/农业农村部农产品质量安全风险评估实验室,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2019年第11期106-115,共10页Heilongjiang Agricultural Sciences

基　　金：国家科技重大专项子课题(2018ZX08015001-003)

摘　　要：为改进我国食品安全监测的方法,采用QuEChERS净化方法结合超高效液相色谱-串联质谱(UPLC-MS/MS)对玉米中的真菌毒素进行检测,建立了玉米中共17种真菌毒素的测定方法。结果表明:17种毒素的线性相关系数(R 2)均不小于0.994,检出限为0.1~20.0μg·kg-1,加标回收率为70.76%~115.14%,相对标准偏差为2.21%~11.34%。该方法具有快速、准确、提取效率高、净化效果好、回收率高、准确灵敏等优点,此方法可适用于玉米和食品中17种真菌毒素的快速检测。In order to improve the method of food safety monitoring in China,QuEChERS purification method combined with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to detect mycotoxins in maize,and a method for determination of 17 mycotoxins in maize was established.The results showed that the linear correlation coefficient(R 2)of 17 toxins was not less than 0.994,the detection limit was 0.1~20.0μg·kg-1,the recoveries were 70.76%~115.14%,and the relative standard deviation was 2.21%~11.34%.The method has the advantages of fast,accurate,high extraction efficiency,good purification effect,high recovery rate,accuracy and sensitivity,and the method can be applied to the rapid detection of 17 mycotoxins in maize and food.

关 键 词：真菌毒素 QUECHERS 超高效液相色谱-串联质谱 玉米 食品 

分 类 号：O65[理学—分析化学]